Construction of cardiac tissue rings using a magnetic tissue fabrication technique.

Construction of cardiac tissue rings using a magnetic tissue fabrication technique.
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DOI:
10.3390/ijms11082910
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发表时间:
2010-08-10
影响因子:
5.6
通讯作者:
Kamihira M
Kamihira M
中科院分区:
生物学2区
文献类型:
--
作者:
Akiyama H;Ito A;Sato M;Kawabe Y;Kamihira M

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在这里,我们应用基于磁力的组织工程技术的心脏组织制造。将细胞外基质前体和用磁性纳米颗粒标记的心肌细胞的混合物加入到含有中心聚碳酸酯圆柱体的孔中。通过使用磁体,将细胞吸引到孔的底部并使其形成细胞层。在培养过程中,细胞层向圆柱体收缩,导致形成具有多层细胞结构和收缩特性的环形组织。这些结果表明,磁性组织制造是一种很有前途的心脏组织工程方法。
Here we applied a magnetic force-based tissue engineering technique to cardiac tissue fabrication. A mixture of extracellular matrix precursor and cardiomyocytes labeled with magnetic nanoparticles was added into a well containing a central polycarbonate cylinder. With the use of a magnet, the cells were attracted to the bottom of the well and allowed to form a cell layer. During cultivation, the cell layer shrank towards the cylinder, leading to the formation of a ring-shaped tissue that possessed a multilayered cell structure and contractile properties. These results indicate that magnetic tissue fabrication is a promising approach for cardiac tissue engineering.
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