Adrenaline stimulates glucagon secretion in pancreatic A-cells by increasing the Ca2+ current and the number of granules close to the L-type Ca2+ channels.
Adrenaline stimulates glucagon secretion in pancreatic A-cells by increasing the Ca2+ current and the number of granules close to the L-type Ca2+ channels.
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肾上腺素通过增加 Ca2+ 电流和靠近 L 型 Ca2+ 通道的颗粒数量来刺激胰腺 A 细胞的胰高血糖素分泌。
DOI：
10.1085/jgp.110.3.217
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发表时间：
1997-09
影响因子：
3.8
通讯作者：
Rorsman, P
中科院分区：
文献类型：
--
作者：
Gromada, J;Bokvist, K;Ding, WG;Barg, S;Buschard, K;Renstrom, E;Rorsman, P
我们监测了电活动，电压门控钙电流，和胞吐在单个大鼠胰高血糖素分泌胰腺A细胞。A细胞是可电兴奋的，并产生自发的Na+和Ca 2+依赖性动作电位。在基础条件下，胞吐作用与通过ω-芋螺毒素-GVIA-敏感性（N-型）Ca 2+通道的Ca 2+内流紧密相关。用肾上腺素（通过β-肾上腺素能受体）或毛喉素刺激A细胞，产生了大于四倍的PKA依赖性增强去极化诱发的胞吐作用。这种胞吐作用的增强是由于通过L-型Ca 2+通道的Ca 2+内流增强50%，该作用占总刺激作用的<30%。剩余的70%的刺激是由于颗粒动员的加速，导致L-型Ca 2+通道附近容易释放的颗粒的数量增加了5倍。
We have monitored electrical activity, voltage-gated Ca2+ currents, and exocytosis in single rat glucagon-secreting pancreatic A-cells. The A-cells were electrically excitable and generated spontaneous Na+- and Ca2+-dependent action potentials. Under basal conditions, exocytosis was tightly linked to Ca2+ influx through ω-conotoxin-GVIA–sensitive (N-type) Ca2+ channels. Stimulation of the A-cells with adrenaline (via β-adrenergic receptors) or forskolin produced a greater than fourfold PKA-dependent potentiation of depolarization-evoked exocytosis. This enhancement of exocytosis was due to a 50% enhancement of Ca2+ influx through L-type Ca2+ channels, an effect that accounted for <30% of the total stimulatory action. The remaining 70% of the stimulation was attributable to an acceleration of granule mobilization resulting in a fivefold increase in the number of readily releasable granules near the L-type Ca2+ channels.
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影响因子：
5.5
作者：
AMMALA, C;ELIASSON, L;RORSMAN, P
通讯作者：
RORSMAN, P
DOI：
10.1006/bbrc.1995.1387
发表时间：
1995-03-17
影响因子：
3.1
作者：
BERTS, A;GYLFE, E;HELLMAN, B
通讯作者：
HELLMAN, B
影响因子：
4.5
作者：
LOPEZ, MG;ALBILLOS, A;ARTALEJO, AR
通讯作者：
ARTALEJO, AR
影响因子：
4.8
作者：
PIPELEERS, DG;SCHUIT, FC;VANDEWINKEL, M
通讯作者：
VANDEWINKEL, M
影响因子：
16.2
作者：
Renstrom, E;Ding, WG;Rorsman, P
通讯作者：
Rorsman, P