Aggregation, Release and Desensitization Induced in Platelets from Five Species by Platelet Activating Factor (PAF)
Aggregation, Release and Desensitization Induced in Platelets from Five Species by Platelet Activating Factor (PAF)
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血小板激活因子 (PAF) 诱导五种血小板聚集、释放和脱敏
DOI：
10.1055/s-0038-1657363
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发表时间：
1983
影响因子：
6.7
通讯作者：
R. Levin
中科院分区：
文献类型：
--
作者：
D. Cargill;D. Cohen;R. Valen;J. Klimek;R. Levin
在人、灵长类动物、豚鼠、兔和大鼠富血小板血浆（PRP）中研究了PAF诱导的聚集和致密颗粒释放（ATP释放）。豚鼠PRP对PAF最敏感，其次是兔，然后是人。当暴露于PAF时，大鼠和灵长类动物的PRP没有聚集或释放。在豚鼠和兔PRP中，释放与聚集反应无关。在人PRP中，高浓度PAF（5 × 10- 7 M）引起最大聚集和双相ATP释放，而低浓度PAF（3×10- 8 M）引起双相聚集和一相ATP释放伴随第二相聚集。PAF诱导的豚鼠和兔PRP聚集和ATP释放依赖于Ca ~（++）水平，而人血小板的反应在一定阈值浓度以上与Ca ~（++）水平相对无关。与PAF聚集在一起的三种PRP也表现出脱敏现象。
Summary PAF-induced aggregation and dense granule release (ATP release) were investigated in human, primate, guinea pig, rabbit and rat platelet-rich plasma (PRP). Guinea pig PRP was most sensitive to PAF, followed by rabbit and then human. PRP from rats and primates did not aggregate or release when exposed to PAF. In guinea pig and rabbit PRP, release was independent of aggregatory response. In human PRP high doses of PAF (5 × 10-7M) caused maximum aggregation and a biphasic ATP release, whilst lower concentrations (3×10-8M) induced biphasic aggregation and one phase of ATP release concomitant with the second phase of aggregation. Aggregation and ATP release induced by PAF in guinea pig and rabbit PRP is dependent upon Ca++ levels, whilst the response of human platelets is relatively independent of the Ca++ level above a certain threshold concentration. The PRP from the three species which aggregated with PAF also demonstrate the phenomenon of desensitization.
DOI：
10.1172/jci110108
发表时间：
1981
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
McManus,LM;Hanahan,DJ;Pinckard,RN
通讯作者：
Pinckard,RN
乙酰甘油醚磷酸胆碱的分子病理学：与血小板激活因子结构和功能同一性的证据。
DOI：
--
发表时间：
1980
期刊：
Journal of the Reticuloendothelial Society
影响因子：
--
作者：
Pinckard,RN;McManus,LM;Demopoulos,CA;Halonen,M;Clark,PO;Shaw,JO;Kniker,WT;Hanahan,DJ
通讯作者：
Hanahan,DJ