Implementation of Epigenetic Variation in Sorghum Selection and Implications for Crop Resilience Breeding.
Implementation of Epigenetic Variation in Sorghum Selection and Implications for Crop Resilience Breeding.
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DOI：
10.3389/fpls.2021.798243
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发表时间：
2021
影响因子：
5.6
通讯作者：
Mackenzie SA
中科院分区：
文献类型：
--
作者：
Ketumile D;Yang X;Sanchez R;Kundariya H;Rajewski J;Dweikat IM;Mackenzie SA
作物恢复力和产量稳定性是粮食安全所必需的复杂性状。高粱是一种重要的粮食作物，表现出对干旱的自然适应能力和边际土地生产潜力。我们已经开发了高粱系在Tx430遗传背景抑制MSH1的表达作为一种手段，诱导从头表观遗传变异，并已使用这些材料来评估植物生长活力的变化。在两个不同的环境中的植物杂交和选择揭示了来自MSH1操纵的表型可塑性的特征。在没有选择的情况下，将表观遗传变异引入等基因高粱群体，在理想的田间条件下导致产量增加10%，在极端低氮条件下增加20%。然而，结合早期选择放大这些结果，在理想条件下增产36%，在边际田条件下增产64%。有趣的是，最好的结果是通过选择中等性能的早期生产线，而不是最高性能的。数据还表明，表观遗传变异产生的表型可塑性对环境变异的反应是不一致的，但有助于减少基因型×环境的相互作用。MSH1衍生的生长活力似乎与增强的幼苗根系生长和生长素反应途径的表达改变有关，植物表现出耐寒性的证据，与以前在拟南芥中观察到的特征一致。这些数据意味着MSH1系统在植物物种中是保守的，指出了平行模式植物研究的价值，以帮助设计有效的植物选择策略，用于多种作物的表观遗传育种。
Crop resilience and yield stability are complex traits essential for food security. Sorghum bicolor is an important grain crop that shows promise for its natural resilience to drought and potential for marginal land production. We have developed sorghum lines in the Tx430 genetic background suppressed for MSH1 expression as a means of inducing de novo epigenetic variation, and have used these materials to evaluate changes in plant growth vigor. Plant crossing and selection in two distinct environments revealed features of phenotypic plasticity derived from MSH1 manipulation. Introduction of an epigenetic variation to an isogenic sorghum population, in the absence of selection, resulted in 10% yield increase under ideal field conditions and 20% increase under extreme low nitrogen conditions. However, incorporation of early-stage selection amplified these outcomes to 36% yield increase under ideal conditions and 64% increase under marginal field conditions. Interestingly, the best outcomes were derived by selecting mid-range performance early-generation lines rather than highest performing. Data also suggested that phenotypic plasticity derived from the epigenetic variation was non-uniform in its response to environmental variability but served to reduce genotype × environment interaction. The MSH1-derived growth vigor appeared to be associated with enhanced seedling root growth and altered expression of auxin response pathways, and plants showed evidence of cold tolerance, features consistent with observations made previously in Arabidopsis. These data imply that the MSH1 system is conserved across plant species, pointing to the value of parallel model plant studies to help devise effective plant selection strategies for epigenetic breeding in multiple crops.
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