Comparative RNA-Seq analysis reveals genes associated with masculinization in female Cannabis sativa.
Comparative RNA-Seq analysis reveals genes associated with masculinization in female Cannabis sativa.
复制标题
DOI：
10.1007/s00425-020-03522-y
复制
发表时间：
2021-01-04
期刊：
影响因子：
4.3
通讯作者：
Mahmoud SS
中科院分区：
文献类型：
--
作者：
Adal AM;Doshi K;Holbrook L;Mahmoud SS
使用RNA分析，我们确定了几个银硫代硫酸盐诱导的基因，可能控制雌性大麻植物的雄性化。遗传雌性大麻植物通常具有雌花，但可以响应环境和发育线索而发育雄花。为了阐明C. sativa植物，我们开发了产生雌花和化学诱导的雄花的遗传雌性品系。此外，我们进行了RNA-Seq测定，旨在鉴定负责雌花发育的差异表达基因。结果显示，有超过10，500个差异表达的基因，其中约200个可能控制雌性大麻植物的雄性化。这些基因包括转录因子和参与雄性器官的其它基因（即，花药和花粉）发育，以及参与植物激素信号传导和雄性偏向表型的基因。通过qPCR测定进一步验证这些基因中的15个的表达，证实与RNA-Seq数据的表达模式相似。这些基因将有助于了解在同一植物中产生两种性别类型的花的易感植物，并帮助育种者通过有针对性的育种和植物生物技术来调节雌性植物的雄性化。在线版本包含补充材料，可通过10.1007/s 00425 -020-03522-y获得。
Using RNA profiling, we identified several silver thiosulfate-induced genes that potentially control the masculinization of female Cannabis sativa plants. Genetically female Cannabis sativa plants normally bear female flowers, but can develop male flowers in response to environmental and developmental cues. In an attempt to elucidate the molecular elements responsible for sex expression in C. sativa plants, we developed genetically female lines producing both female and chemically-induced male flowers. Furthermore, we carried out RNA-Seq assays aimed at identifying differentially expressed genes responsible for male flower development in female plants. The results revealed over 10,500 differentially expressed genes, of which around 200 potentially control masculinization of female cannabis plants. These genes include transcription factors and other genes involved in male organ (i.e., anther and pollen) development, as well as genes involved in phytohormone signalling and male-biased phenotypes. The expressions of 15 of these genes were further validated by qPCR assay confirming similar expression patterns to that of RNA-Seq data. These genes would be useful for understanding predisposed plants producing flowers of both sex types in the same plant, and help breeders to regulate the masculinization of female plants through targeted breeding and plant biotechnology. The online version contains supplementary material available at 10.1007/s00425-020-03522-y.
登录
查看更多内容
DOI：
10.1093/bioinformatics/btr490
发表时间：
2011-11-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Howe EA;Sinha R;Schlauch D;Quackenbush J
通讯作者：
Quackenbush J
影响因子：
5.3
作者：
Kram BW;Xu WW;Carter CJ
通讯作者：
Carter CJ
影响因子：
11.6
作者：
Kater, MM;Colombo, L;Angenent, GC
通讯作者：
Angenent, GC
影响因子：
5.9
作者：
Bernstein, Nirit;Gorelick, Jonathan;Koch, Sraya
通讯作者：
Koch, Sraya
影响因子：
14.9
作者：
Bouché F;Lobet G;Tocquin P;Périlleux C
通讯作者：
Périlleux C