Uncovering the Arabidopsis thaliana nectary transcriptome: investigation of differential gene expression in floral nectariferous tissues.
Uncovering the Arabidopsis thaliana nectary transcriptome: investigation of differential gene expression in floral nectariferous tissues.
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DOI：
10.1186/1471-2229-9-92
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发表时间：
2009-07-15
影响因子：
5.3
通讯作者：
Carter CJ
中科院分区：
文献类型：
--
作者：
Kram BW;Xu WW;Carter CJ
许多开花植物通过提供花蜜来吸引传粉者。值得注意的是，与蜜腺发育有关的分子事件，产生花蜜的器官，以及花蜜本身的合成和分泌，人们知之甚少。事实上，到目前为止，还没有基因被证明能直接影响花蜜的新生产或质量。为了解决这一知识空白，ATH1 Affymetrix®基因芯片阵列被用于系统地研究拟南芥花蜜转录组，以确定可能参与花蜜产生的基因和途径。在这项研究中，我们发现了大量的基因在分泌型外侧蜜腺和非分泌型内侧蜜腺组织之间，以及成熟型外侧蜜腺（花后）和未成熟型外侧蜜腺（花前）之间的差异表达。蜜腺内的表达也与13个非蜜腺参比组织进行了比较，其中270个基因被鉴定为在蜜腺中显著上调。通过RT - PCR验证了14个蜜糖富集基因的表达模式。在研究上调基因的功能群时，与参比组织相比，涉及基因调控、碳水化合物代谢和脂质代谢的途径在蜜腺中特别丰富。发现了大量在蜜腺中优先表达的基因，以及蜜腺类型和发育阶段之间的基因。提出了关于花蜜产生及其调控机制的几个假设，并为反向遗传学方法确定花蜜合成和分泌的分子机制提供了一个起点。
Many flowering plants attract pollinators by offering a reward of floral nectar. Remarkably, the molecular events involved in the development of nectaries, the organs that produce nectar, as well as the synthesis and secretion of nectar itself, are poorly understood. Indeed, to date, no genes have been shown to directly affect the de novo production or quality of floral nectar. To address this gap in knowledge, the ATH1 Affymetrix® GeneChip array was used to systematically investigate the Arabidopsis nectary transcriptome to identify genes and pathways potentially involved in nectar production. In this study, we identified a large number of genes differentially expressed between secretory lateral nectaries and non-secretory median nectary tissues, as well as between mature lateral nectaries (post-anthesis) and immature lateral nectaries (pre-anthesis). Expression within nectaries was also compared to thirteen non-nectary reference tissues, from which 270 genes were identified as being significantly upregulated in nectaries. The expression patterns of 14 nectary-enriched genes were also confirmed via RT PCR. Upon looking into functional groups of upregulated genes, pathways involved in gene regulation, carbohydrate metabolism, and lipid metabolism were particularly enriched in nectaries versus reference tissues. A large number of genes preferentially expressed in nectaries, as well as between nectary types and developmental stages, were identified. Several hypotheses relating to mechanisms of nectar production and regulation thereof are proposed, and provide a starting point for reverse genetics approaches to determine molecular mechanisms underlying nectar synthesis and secretion.
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影响因子：
5.1
作者：
Carter, C;Thornburg, RW
通讯作者：
Thornburg, RW
影响因子：
5.1
作者：
Carter, CJ;Thornburg, RW
通讯作者：
Thornburg, RW
影响因子：
3.8
作者：
ECROYD, CE;FRANICH, RA;STEWARD, D
通讯作者：
STEWARD, D
影响因子：
3
作者：
FAHN, A
通讯作者：
FAHN, A
影响因子：
5.3
作者：
Evrard, Alexandre;Ndatimana, Theogene;Eulgem, Thomas
通讯作者：
Eulgem, Thomas