Diabetes-associated microbiota in fa/fa rats is modified by Roux-en-Y gastric bypass.
Diabetes-associated microbiota in fa/fa rats is modified by Roux-en-Y gastric bypass.
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Roux-en-Y 胃绕道术改变了 fa/fa 大鼠中与糖尿病相关的微生物群。
DOI：
10.1038/ismej.2017.70
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发表时间：
2017-09
期刊：
影响因子：
--
通讯作者：
Bäckhed F
中科院分区：
文献类型：
--
作者：
Arora T;Seyfried F;Docherty NG;Tremaroli V;le Roux CW;Perkins R;Bäckhed F
Roux-en-Y胃旁路术（RYGB）和十二指肠空肠旁路术（DJB）是两种不同形式的减肥手术，与改善葡萄糖耐量相关，但尚不清楚肠道微生物群是否有助于这种效果。在这里，我们使用fa/fa大鼠作为葡萄糖耐量受损的模型来研究（i）fa/fa和非糖尿病fa/+大鼠之间的微生物群是否不同;（ii）fa/fa大鼠的微生物群是否受到RYGB和/或DJB的影响;以及（iii）手术诱导的微生物群改变是否有助于葡萄糖代谢。与非糖尿病fa/+大鼠相比，我们观察到糖尿病fa/fa大鼠小肠中厚壁菌门（特别是动物乳杆菌和罗伊氏乳杆菌）的显著扩张。RYGB处理的fa/fa大鼠，而不是DJB处理的fa/fa大鼠，在回肠和下L中表现出更大的微生物群多样性。animalis和L.在所有肠段中，与假手术的FA/FA大鼠相比，它们的罗伊氏菌丰度增加，并且它们的微生物群组成类似于未手术的FA/+大鼠。为了研究RYGB相关微生物群改变的功能作用，我们将微生物群从假手术和RYGB治疗的fa/fa大鼠转移到无菌小鼠。RYGB处理的大鼠的代谢表型没有通过回肠微生物群的移植而转移。相比之下，接受来自RYGB的盲肠微生物群的小鼠与假手术大鼠相比，餐后峰值葡萄糖水平较低。因此，fa/fa大鼠中糖尿病相关的微生物群改变可以通过RYGB进行修改，并且盲肠微生物群的修改可能部分有助于RYGB后葡萄糖耐量的改善。
Roux-en-Y gastric bypass (RYGB) and duodenal jejunal bypass (DJB), two different forms of bariatric surgery, are associated with improved glucose tolerance, but it is not clear whether the gut microbiota contributes to this effect. Here we used fa/fa rats as a model of impaired glucose tolerance to investigate whether (i) the microbiota varies between fa/fa and nondiabetic fa/+ rats; (ii) the microbiota of fa/fa rats is affected by RYGB and/or DJB; and (iii) surgically induced microbiota alterations contribute to glucose metabolism. We observed a profound expansion of Firmicutes (specifically, Lactobacillus animalis and Lactobacillus reuteri) in the small intestine of diabetic fa/fa compared with nondiabetic fa/+ rats. RYGB-, but not DJB-, treated fa/fa rats exhibited greater microbiota diversity in the ileum and lower L. animalis and L. reuteri abundance compared with sham-operated fa/fa rats in all intestinal segments, and their microbiota composition resembled that of unoperated fa/+ rats. To investigate the functional role of RYGB-associated microbiota alterations, we transferred microbiota from sham- and RYGB-treated fa/fa rats to germ-free mice. The metabolic phenotype of RYGB-treated rats was not transferred by the transplant of ileal microbiota. In contrast, postprandial peak glucose levels were lower in mice that received cecal microbiota from RYGB- versus sham-operated rats. Thus, diabetes-associated microbiota alterations in fa/fa rats can be modified by RYGB, and modifications in the cecal microbiota may partially contribute to improved glucose tolerance after RYGB.
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影响因子：
3.2
作者：
Kindel, Tammy L.;Yoder, Stephanie M.;Tso, Patrick
通讯作者：
Tso, Patrick
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5.3
作者：
Fak, Frida;Tremaroli, Valentina;Backhed, Fredrik
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Backhed, Fredrik
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Schloss, Patrick D.
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作者：
Graessler, J.;Bornstein, T. D.;Bornstein, S. R.
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