Immunogenicity Analysis of the Recombinant Plasmodium falciparum Surface-Related Antigen in Mice.
Immunogenicity Analysis of the Recombinant Plasmodium falciparum Surface-Related Antigen in Mice.
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重组恶性疟原虫表面相关抗原在小鼠体内的免疫原性分析。
DOI：
10.3390/pathogens11050550
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发表时间：
2022-05-07
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
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作者：
恶性疟原虫主要分布在世界热带和亚热带地区，其严重程度引起了广泛关注。恶性疟原虫表面相关抗原（PfSRA）是一种新型蛋白，具有结构和功能特点，可作为一种候选疟疾疫苗；然而，关于其免疫原性的信息有限。我们在大肠杆菌系统中表达了三个重组PfSRA片段，并进一步分析了其免疫原性。结果显示，rpfsra免疫小鼠产生了高终点效价（1:10 000 ~ 1:5 12万）的特异性抗体和亲和抗体（即rPfSRA-F1a（97.70%）、rPfSRA-F2a（69.62%）和rPfSRA-F3a(91.87%)）。此外，免疫小鼠血清均能识别原生PfSRA和重组PfSRA， rPfSRA抗体能抑制恶性疟原虫对红细胞的侵袭，且在体外呈剂量依赖性。本研究证实PfSRA具有免疫原性，特别是保守区n端F1a，进一步支持其作为恶性疟原虫的候选疫苗。
Plasmodium falciparum, mainly distributed in tropical and subtropical regions of the world, has received widespread attention owing to its severity. As a novel protein, P. falciparum surface-related antigen (PfSRA) has the structural and functional characteristics to be considered as a malaria vaccine candidate; however, limited information is available on its immunogenicity. Here, we expressed three fragments of recombinant PfSRA in an Escherichia coli system and further analyzed its immunogenicity. The results showed that rPfSRA-immunized mice produced specific antibodies with high endpoint titers (1:10,000 to 1:5,120,000) and affinity antibodies (i.e., rPfSRA-F1a (97.70%), rPfSRA-F2a (69.62%), and rPfSRA-F3a (91.87%)). In addition, the sera of immunized mice recognized both the native PfSRA and recombinant PfSRA, the rPfSRA antibodies inhibited the invasion of P. falciparum into the erythrocytes, and they were dose-dependent in vitro. This study confirmed PfSRA could be immunogenic, especially the F1a at the conserved region N-terminal and provided further support for it as a vaccine candidate against P. falciparum.
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一种新型的恶性疟原虫相关的粘附素通过唾液酸依赖性途径介导红细胞侵袭。
影响因子：
4.6
作者：
Anand G;Reddy KS;Pandey AK;Mian SY;Singh H;Mittal SA;Amlabu E;Bassat Q;Mayor A;Chauhan VS;Gaur D
通讯作者：
Gaur D
DOI：
10.1084/jem.186.10.1689
发表时间：
1997-11-17
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Guevara Patiño JA;Holder AA;McBride JS;Blackman MJ
通讯作者：
Blackman MJ
影响因子：
15.8
作者：
Druilhe P;Spertini F;Soesoe D;Corradin G;Mejia P;Singh S;Audran R;Bouzidi A;Oeuvray C;Roussilhon C
通讯作者：
Roussilhon C
影响因子：
7
作者：
Gilson, Paul R.;Nebl, Thomas;Crabb, Brendan S.
通讯作者：
Crabb, Brendan S.
非洲输入中国恶性疟原虫表面相关抗原（SRA）遗传多样性分析。
影响因子：
3.7
作者：
Yang B;Liu H;Xu QW;Sun YF;Xu S;Zhang H;Tang JX;Zhu GD;Liu YB;Cao J;Cheng Y
通讯作者：
Cheng Y