Genetic Diversity Analysis of Surface-Related Antigen (SRA) in Plasmodium falciparum Imported From Africa to China.
Genetic Diversity Analysis of Surface-Related Antigen (SRA) in Plasmodium falciparum Imported From Africa to China.
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非洲输入中国恶性疟原虫表面相关抗原（SRA）遗传多样性分析。
DOI：
10.3389/fgene.2021.688606
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发表时间：
2021
影响因子：
3.7
通讯作者：
Cheng Y
中科院分区：
文献类型：
--
作者：
Yang B;Liu H;Xu QW;Sun YF;Xu S;Zhang H;Tang JX;Zhu GD;Liu YB;Cao J;Cheng Y
恶性疟原虫表面相关抗原（SRA）位于配子体和裂殖子表面，具有多阶段疫苗开发的潜在靶点的结构和功能特征。然而，关于pfsra的遗传多态性的信息很少。为了通过表征sra序列来确定恶性疟原虫的遗传变异程度，从2015年至2019年期间从非洲12个国家返回中国的农民工中收集了74份恶性疟原虫样本。扩增sra基因的全长并测序。恶性疟原虫sra基因的平均核苷酸间差异度（π）为0.00132，单倍型多样度（Hd）为0.770。pfsra的平均核苷酸差异数（k）为3.049。跨位点的非同义（dN）与同义（dS）取代的比率（dN/dS）为1.365。恶性疟原虫SRA的氨基酸替换可分为35个独特的氨基酸变体。中性试验表明PfSRA的多态性通过正向多样性选择得以维持，表明其作为保护性免疫应答的潜在靶点和疫苗候选物的作用。总体而言，PfSRA抗体的N-末端引起红细胞裂殖子侵入的抑制的能力和在低遗传多样性下的保守氨基酸表明PfSRA的N-末端可以被评估为针对恶性疟原虫感染的疫苗候选物。
Plasmodium falciparum surface-related antigen (SRA) is located on the surfaces of gametocyte and merozoite and has the structural and functional characteristics of potential targets for multistage vaccine development. However, little information is available regarding the genetic polymorphism of pfsra. To determine the extent of genetic variation about P. falciparum by characterizing the sra sequence, 74 P. falciparum samples were collected from migrant workers who returned to China from 12 countries of Africa between 2015 and 2019. The full length of the sra gene was amplified and sequenced. The average pairwise nucleotide diversities (π) of P. falciparum sra gene was 0.00132, and the haplotype diversity (Hd) was 0.770. The average number of nucleotide differences (k) for pfsra was 3.049. The ratio of non-synonymous (dN) to synonymous (dS) substitutions across sites (dN/dS) was 1.365. Amino acid substitutions of P. falciparum SRA could be categorized into 35 unique amino acid variants. Neutrality tests showed that the polymorphism of PfSRA was maintained by positive diversifying selection, which indicated its role as a potential target of protective immune responses and a vaccine candidate. Overall, the ability of the N-terminal of PfSRA antibodies to evoke inhibition of merozoite invasion of erythrocytes and conserved amino acid at low genetic diversity suggest that the N-terminal of PfSRA could be evaluated as a vaccine candidate against P. falciparum infection.
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影响因子：
9.8
作者：
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通讯作者：
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作者：
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通讯作者：
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影响因子：
3.2
作者：
Chu R;Zhang X;Xu S;Chen L;Tang J;Li Y;Chen J;Xuan Y;Zhu G;Cao J;Cheng Y
通讯作者：
Cheng Y