Establishment and characterization of patient-derived tumor xenograft using gastroscopic biopsies in gastric cancer.
Establishment and characterization of patient-derived tumor xenograft using gastroscopic biopsies in gastric cancer.
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使用胃癌胃镜活检建立患者来源的肿瘤异种移植物并对其进行表征
DOI：
10.1038/srep08542
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发表时间：
2015-02-25
影响因子：
4.6
通讯作者：
Shen L
中科院分区：
文献类型：
--
作者：
Zhu Y;Tian T;Li Z;Tang Z;Wang L;Wu J;Li Y;Dong B;Li Y;Li N;Zou J;Gao J;Shen L
The patient-derived tumor xenograft (PDTX) model has become the most realistic model for preclinical studies. PDTX models of gastric cancer using surgical tissues are reported occasionally; however, the PDTX models using gastroscopic biopsies, which are best for evaluating new drugs, are unreported. In our study, a total of 185 fresh gastroscopic biopsies of gastric cancer were subcutaneously transplanted into NOD/SCID (Nonobese Diabetic/Severe Combined Immunodeficiency) mice. Sixty-three PDTX models were successfully established (34.1%, 63/185) and passaged to maintain tumorsin vivoand the mean latency period of xenografts was 65.86 ± 32.84 days (11–160 days). Biopsies of prior chemotherapy had a higher transplantation rate (52.1%, 37/71) than biopsies after chemotherapy (21.9%, 25/114;P= 0.000). No differences were found between the latency period of xenografts and characteristics of patients. The pathological and molecular features of PDTX as well as chemosensitivity were highly consistent with those of primary tumors of patients. The genetic characteristics were stable during passaging of PDTX models. In summary PDTX models using gastroscopic biopsies in gastric cancer were demonstrated for the first time and the biological characteristics of the PDTX models were highly consistent with patients, which provided the best preclinical study platform for gastric cancer.
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影响因子：
64.8
作者：
通讯作者：
--
DOI：
10.1158/1078-0432.ccr-12-3140
发表时间：
2013-08-01
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Spreafico A;Tentler JJ;Pitts TM;Tan AC;Gregory MA;Arcaroli JJ;Klauck PJ;McManus MC;Hansen RJ;Kim J;Micel LN;Selby HM;Newton TP;McPhillips KL;Gustafson DL;Degregori JV;Messersmith WA;Winn RA;Eckhardt SG
通讯作者：
Eckhardt SG
影响因子：
17.1
作者：
Sivanand S;Peña-Llopis S;Zhao H;Kucejova B;Spence P;Pavia-Jimenez A;Yamasaki T;McBride DJ;Gillen J;Wolff NC;Morlock L;Lotan Y;Raj GV;Sagalowsky A;Margulis V;Cadeddu JA;Ross MT;Bentley DR;Kabbani W;Xie XJ;Kapur P;Williams NS;Brugarolas J
通讯作者：
Brugarolas J
影响因子：
11.2
作者：
Daniel VC;Marchionni L;Hierman JS;Rhodes JT;Devereux WL;Rudin CM;Yung R;Parmigiani G;Dorsch M;Peacock CD;Watkins DN
通讯作者：
Watkins DN
影响因子：
3.8
作者：
Linnebacher M;Maletzki C;Ostwald C;Klier U;Krohn M;Klar E;Prall F
通讯作者：
Prall F