Cryopreservation of human colorectal carcinomas prior to xenografting.
Cryopreservation of human colorectal carcinomas prior to xenografting.
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DOI：
10.1186/1471-2407-10-362
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发表时间：
2010-07-08
期刊：
影响因子：
3.8
通讯作者：
Prall F
中科院分区：
文献类型：
--
作者：
Linnebacher M;Maletzki C;Ostwald C;Klier U;Krohn M;Klar E;Prall F
结直肠癌(CRC)的分子异质性是公认的，这形成了在给药一些新的靶向治疗之前需要进行分子检测的理论基础，这些治疗现在正迅速进入临床。至少对于临床研究，甚至对于未来常规的个体化肿瘤治疗，增殖患者的结直肠癌组织可能是非常必要的，以进行详细的分子、生化或功能分析。然而，复杂的后勤需要外科、病理学、实验室研究人员和动物护理机构之间的密切联系，这是这方面的一个主要缺点。在此，我们描述并评估了一种非常简单的结直肠癌组织异种移植前的冷冻保存程序，它将大大降低这一逻辑复杂性。将收集的48个CRC移植到手术后新鲜(N=23)或冷冻后(N=31；长达643天)免疫缺陷小鼠的皮下。冷冻保存后的取样率令人满意(71%)，但略低于手术后新鲜肿瘤组织(74%)，但这一差异没有统计学意义。冷冻保存的已建立的异种移植物(N=11)再次移植均成功。值得注意的是，在这个系列中，所有主要的结直肠癌分子类型都成功地异种移植，即使在冷冻保存之后也是如此。我们的程序促进了临床结直肠癌标本的收集、长期存储和传播(甚至从不同的中心)，以用于新疗法的(临床前)研究或基础研究。
Molecular heterogeneity of colorectal carcinoma (CRC) is well recognized, forming the rationale for molecular tests required before administration of some of the novel targeted therapies that now are rapidly entering the clinics. For clinical research at least, but possibly even for future individualized tumor treatment on a routine basis, propagation of patients' CRC tissue may be highly desirable for detailed molecular, biochemical or functional analyses. However, complex logistics requiring close liaison between surgery, pathology, laboratory researchers and animal care facilities are a major drawback in this. We here describe and evaluate a very simple cryopreservation procedure for colorectal carcinoma tissue prior to xenografting that will considerably reduce this logistic complexity. Fourty-eight CRC collected ad hoc were xenografted subcutaneously into immunodeficient mice either fresh from surgery (N = 23) or after cryopreservation (N = 31; up to 643 days). Take rates after cryopreservation were satisfactory (71%) though somewhat lower than with tumor tissues fresh from surgery (74%), but this difference was not statistically significant. Re-transplantation of cryopreserved established xenografts (N = 11) was always successful. Of note, in this series, all of the major molecular types of CRC were xenografted successfully, even after cryopreservation. Our procedure facilitates collection, long-time storage and propagation of clinical CRC specimens (even from different centres) for (pre)clinical studies of novel therapies or for basic research.
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影响因子：
2.3
作者：
Jass, Jeremy R.
通讯作者：
Jass, Jeremy R.
DOI：
10.1111/j.1440-1746.1991.tb00867.x
发表时间：
1991-07-01
影响因子：
4.1
作者：
FIELDING, LP;ARSENAULT, PA;NEWLAND, RC
通讯作者：
NEWLAND, RC
影响因子：
3.6
作者：
Bocsi, J;Zalatnai, A
通讯作者：
Zalatnai, A
影响因子：
5.2
作者：
Ostwald, Christiane;Linnebacher, Michael;Prall, Friedrich
通讯作者：
Prall, Friedrich
影响因子：
3.4
作者：
Freeman, Daniel J.;Juan, Todd;Amado, Rafael G.
通讯作者：
Amado, Rafael G.