chlB requirement for chlorophyll biosynthesis under short photoperiod in Marchantia polymorpha L.
chlB requirement for chlorophyll biosynthesis under short photoperiod in Marchantia polymorpha L.
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DOI：
10.1093/gbe/evu045
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发表时间：
2014-03
影响因子：
3.3
通讯作者：
Nishimura Y
中科院分区：
文献类型：
--
作者：
Ueda M;Tanaka A;Sugimoto K;Shikanai T;Nishimura Y
叶绿素在所有光合生物的能量吸收和传递以及反应中心的电荷分离中起着关键作用。在叶绿素的生物合成步骤中，只有一个酶促还原原叶绿素（Pchlide）为叶绿素（Chlide）的步骤是由陆地植物的核和叶绿体编码的基因介导的。许多植物在细胞核和叶绿体基因组中分别编码光依赖性Pchlide还原酶（LPOR）和光非依赖性Pchlide还原酶（DPOR）基因。在陆生植物的多样化过程中，Pchlide到Chlide的还原步骤已经变得完全依赖于LPOR，并且DPOR基因已经从叶绿体基因组中丢失。目前还不清楚为什么DPOR在某些陆生植物中持续存在，它们是如何在陆生植物多样化过程中从叶绿体基因组中消除的，以及在什么环境条件下需要DPOR。我们证明了DPOR在地钱（地钱L.）在叶绿素合成中起重要作用。在建立了苔类植物的质体转化系统后，我们破坏了编码M.多形叶绿体基因组。形态学和叶绿素含量的chlB突变体生长在不同的光周期分析表明，DPOR是特别需要在短日照条件下的叶绿素生物合成。我们的研究结果表明，光周期的形式的环境条件是一个重要的因素，决定损失或保留的叶绿体编码的基因介导Pchlide还原为Chlide。
Chlorophylls (Chls) play pivotal roles in energy absorption and transduction and also in charge separation in reaction centers in all photosynthetic organisms. In Chl biosynthesis steps, only a step for the enzymatic reduction of protochlorophyllide (Pchlide) to chlorophyllide (Chlide) is mediated by both nuclear- and chloroplast-encoded genes in land plants. Many plants encode the genes for light-dependent Pchlide reductase (LPOR) and light-independent Pchlide reductase (DPOR) in the nucleus and chloroplast genome, respectively. During the diversification of land plants, the reduction step of Pchlide to Chlide has become solely dependent on LPOR, and the genes for DPOR have been lost from chloroplast genome. It remains unclear why DPOR persists in some land plants, how they were eliminated from chloroplast genomes during the diversification of land plants, and under what environmental conditions DPOR was required. We demonstrate that DPOR is functional in liverwort (Marchantia polymorpha L.) and plays an important role in Chl biosynthesis. Having established a plastid transformation system in liverwort, we disrupted chlB, which encodes a subunit of DPOR in the M. polymorpha chloroplast genome. Morphological and Chl content analysis of a chlB mutant grown under different photoperiods revealed that DPOR is particularly required for Chl biosynthesis under short-day conditions. Our findings suggest that an environmental condition in the form of photoperiod is an important factor that determines the loss or retention of chloroplast-encoded genes mediating Pchlide reduction to Chlide.
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影响因子：
4.9
作者：
Nagata, Nozomi;Tanaka, Ryouichi;Tanaka, Ayumi
通讯作者：
Tanaka, Ayumi
影响因子：
64.8
作者：
OHYAMA, K;FUKUZAWA, H;OZEKI, H
通讯作者：
OZEKI, H
DOI：
10.1073/pnas.182432999
发表时间：
2002-09-17
影响因子：
11.1
作者：
Martin, W;Rujan, T;Penny, D
通讯作者：
Penny, D
影响因子：
4.9
作者：
FUJITA, Y;TAKAHASHI, Y;MATSUBARA, H
通讯作者：
MATSUBARA, H
影响因子：
11.6
作者：
Nagata, N;Tanaka, R;Tanaka, A
通讯作者：
Tanaka, A