Rapid, accurate, and non-invasive measurement of zebrafish axial length and other eye dimensions using SD-OCT allows longitudinal analysis of myopia and emmetropization.
Rapid, accurate, and non-invasive measurement of zebrafish axial length and other eye dimensions using SD-OCT allows longitudinal analysis of myopia and emmetropization.
复制标题
DOI：
10.1371/journal.pone.0110699
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Link BA
中科院分区：
文献类型：
--
作者：
Collery RF;Veth KN;Dubis AM;Carroll J;Link BA
视力屈光不正可能由眼轴长度异常、角膜形状不规则或透镜异常引起。眼长过度增长的原因包括多个遗传基因座和视觉参数。我们评估斑马鱼作为一种潜在的动物模型，用于研究正视化和近视的遗传、细胞和信号基础。利用光谱域光学相干断层扫描（SD-OCT）测量了斑马鱼的眼轴长度和其他尺寸。我们使用眼透镜和身体指标来标准化和比较野生型和lrp 2斑马鱼的眼睛大小和相对屈光不正（观察到的视网膜径向长度和对照之间的差异）。将斑马鱼在黑暗中饲养以评估视觉剥夺对眼睛大小的影响。两个相对测量，眼轴长度体长和眼轴长度透镜直径，被发现准确地归一化不同大小的鱼之间的眼睛大小的比较（R2 = 0.9548，R2 = 0.9921）。 野生型斑马鱼晶状体的光线追踪焦距等于其视网膜半径，而lrp 2眼睛的视网膜半径比焦距长。基因突变（lrp 2）和环境操纵（黑暗饲养）导致眼轴延长。与野生型相比，lrp 2突变体的相对屈光不正为-0.327，与光照饲养的同胞相比，黑暗饲养的野生型鱼类的相对屈光不正为-0.132。因此，斑马鱼的眼睛解剖结构（轴长，透镜半径，视网膜半径）可以快速，准确地测量SD-OCT，促进纵向研究的调节眼睛的生长和正视化。具体地说，与人类近视候选者同源的基因可以在斑马鱼中被修饰、失活或过表达，并且近视致敏条件用于探测基因-环境相互作用。我们的研究为控制眼睛大小和影响屈光不正的遗传贡献的调查提供了基础。
Refractive errors in vision can be caused by aberrant axial length of the eye, irregular corneal shape, or lens abnormalities. Causes of eye length overgrowth include multiple genetic loci, and visual parameters. We evaluate zebrafish as a potential animal model for studies of the genetic, cellular, and signaling basis of emmetropization and myopia. Axial length and other eye dimensions of zebrafish were measured using spectral domain-optical coherence tomography (SD-OCT). We used ocular lens and body metrics to normalize and compare eye size and relative refractive error (difference between observed retinal radial length and controls) in wild-type and lrp2 zebrafish. Zebrafish were dark-reared to assess effects of visual deprivation on eye size. Two relative measurements, ocular axial length to body length and axial length to lens diameter, were found to accurately normalize comparisons of eye sizes between different sized fish (R2 = 0.9548, R2 = 0.9921). Ray-traced focal lengths of wild-type zebrafish lenses were equal to their retinal radii, while lrp2 eyes had longer retinal radii than focal lengths. Both genetic mutation (lrp2) and environmental manipulation (dark-rearing) caused elongated eye axes. lrp2 mutants had relative refractive errors of −0.327 compared to wild-types, and dark-reared wild-type fish had relative refractive errors of −0.132 compared to light-reared siblings. Therefore, zebrafish eye anatomy (axial length, lens radius, retinal radius) can be rapidly and accurately measured by SD-OCT, facilitating longitudinal studies of regulated eye growth and emmetropization. Specifically, genes homologous to human myopia candidates may be modified, inactivated or overexpressed in zebrafish, and myopia-sensitizing conditions used to probe gene-environment interactions. Our studies provide foundation for such investigations into genetic contributions that control eye size and impact refractive errors.
登录
查看更多内容
影响因子：
1.8
作者：
CHARMAN, WN;TUCKER, J
通讯作者：
TUCKER, J
影响因子：
15.9
作者：
Howell, Gareth R.;Soto, Ileana;John, Simon W. M.
通讯作者：
John, Simon W. M.
影响因子：
46.9
作者：
通讯作者：
--
影响因子：
1.8
作者：
Graham, B;Judge, SJ
通讯作者：
Judge, SJ
影响因子：
64.8
作者：
通讯作者：
--