Contrasting patterns of insecticide resistance and knockdown resistance (kdr) in the dengue vectors Aedes aegypti and Aedes albopictus from Malaysia.
Contrasting patterns of insecticide resistance and knockdown resistance (kdr) in the dengue vectors Aedes aegypti and Aedes albopictus from Malaysia.
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DOI：
10.1186/s13071-015-0797-2
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发表时间：
2015-03-25
影响因子：
3.2
通讯作者：
Wondji CS
中科院分区：
文献类型：
--
作者：
Ishak IH;Jaal Z;Ranson H;Wondji CS
了解杀虫剂耐药性的程度、分布和机制是成功开展以杀虫剂为基础的登革热控制干预措施的关键。在这里，我们报告了广泛的登革热媒介埃及伊蚊和白纹伊蚊在马来西亚的抗性概况，并建立了击倒抗性机制的贡献，揭示了这两个物种之间的显著差异。2010年，使用诱蚊产卵器从马来西亚的四个州采集了伊蚊，并使用世卫组织的生物检测方法对六种主要杀虫剂进行了测试。研究了这两个物种的击倒抗性(Kdr)。2010年在槟城、吉隆坡、柔佛巴鲁和哥打巴鲁(1.5<；RR<；3.3)采集的样本中检测到对泰米福斯的中等抗性。在Ae中观察到广泛的多重抗性。埃及伊蚊对拟除虫菊酯、滴滴涕和杀菌威有特别的杀灭作用。来自吉隆坡的蚊子一直具有最高的抗性水平，是唯一对马拉硫磷表现出中等抗性的种群(死亡率为91%)。Ae.的抗性图谱。白纹伊蚊与Ae.埃及伊蚊对拟除虫菊酯完全敏感，但在吉隆坡观察到中等抗性。PBO增效剂分析表明，代谢抗性机制在这两个物种的抗性中发挥了主要作用。在Ae中检测到两个kdR突变，分别为F1534C和V1016G。马来西亚各地都有埃及伊蚊，但在Ae中没有发现这两种突变。白纹伊蚊。此外，在Ae的电压门控钠通道基因上检测到了选择的特征。埃及人，但不是在Ae.白纹伊蚊。1534C等位基因的存在与拟除虫菊酯抗性显著相关，在含有这两个kdr等位基因的个体中观察到了对拟除虫菊酯抗性的相加效应。这项研究的发现将有助于设计和实施成功的以杀虫剂为基础的干预措施。Aegypti和Ae.白纹伊蚊以改善马来西亚各地的登革热控制。本文的在线版本(doi：10.1186/s13071-0150797-2)包含补充材料，授权用户可以使用。
Knowledge on the extent, distribution and mechanisms of insecticide resistance is essential for successful insecticide-based dengue control interventions. Here, we report an extensive resistance profiling of the dengue vectors Aedes aegypti and Aedes albopictus across Malaysia and establish the contribution of knockdown resistance mechanism revealing significant contrast between both species. Aedes mosquitoes were collected from four states in Malaysia in 2010 using ovitraps and tested against six major insecticides using WHO bioassays. Knockdown resistance (kdr) was investigated in both species. A moderate resistance to temephos was detected from samples collected in 2010 in Penang, Kuala Lumpur, Johor Bharu and Kota Bharu (1.5 < RR < 3.3). A widespread and multiple resistances was observed in Ae. aegypti particularly against pyrethroids, DDT and bendiocarb. Mosquitoes from Kuala Lumpur consistently had the highest resistance levels and was the only population showing a moderate resistance to malathion (91% mortality). The resistance profile of Ae. albopictus contrasted to Ae. aegypti with full susceptibility to pyrethroids except in Kuala Lumpur where moderate resistance is observed. PBO synergist assays suggest metabolic resistance mechanisms play a major role in resistance in both species. Two kdr mutations, F1534C and V1016G, were detected in Ae. aegypti across Malaysia but neither of these mutations were found in Ae. albopictus. Additionally, signatures of selection were detected on the Voltage-gated sodium channel gene in Ae. aegypti but not in Ae. albopictus. The presence of the 1534C allele was significantly associated with pyrethroid resistance and an additive effect to pyrethroid resistance was observed in individuals containing both kdr alleles. Findings from this study will help to design and implement successful insecticide-based interventions against Ae. aegypti and Ae. albopictus to improve dengue control across Malaysia. The online version of this article (doi:10.1186/s13071-015-0797-2) contains supplementary material, which is available to authorized users.
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影响因子：
4.9
作者：
Clement, M;Posada, D;Crandall, KA
通讯作者：
Crandall, KA
影响因子：
1.9
作者：
Brengues, C;Hawkes, NJ;Hemingway, J
通讯作者：
Hemingway, J
DOI：
10.1016/s1474-8177(07)07014-3
发表时间：
2007-01-01
期刊：
PERSISTENT ORGANIC POLLUTANTS IN ASIA: SOURCES, DISTRIBUTIONS, TRANSPORT AND FATE
影响因子：
--
作者：
Ibrahim, Md Sani
通讯作者：
Ibrahim, Md Sani
影响因子：
3.8
作者：
Kawada H;Oo SZ;Thaung S;Kawashima E;Maung YN;Thu HM;Thant KZ;Minakawa N
通讯作者：
Minakawa N
DOI：
10.1093/emph/eot012
发表时间：
2013-01
期刊：
Evolution, medicine, and public health
影响因子：
--
作者：
Martins AJ;Brito LP;Linss JG;Rivas GB;Machado R;Bruno RV;Lima JB;Valle D;Peixoto AA
通讯作者：
Peixoto AA