Changes in proteomic profiles in different prostate lobes of male rats throughout growth and development and aging stages of the life span.
Changes in proteomic profiles in different prostate lobes of male rats throughout growth and development and aging stages of the life span.
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DOI：
10.1002/pros.22576
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发表时间：
2013-03
期刊：
影响因子：
2.8
通讯作者：
El-Bayoumy, Karam
中科院分区：
文献类型：
--
作者：
Das, Arunangshu;Bortner, James D., Jr.;Aliaga, Cesar A.;Baker, Aaron;Stanley, Anne;Stanley, Bruce A.;Kaag, Matthew;Richie, John P., Jr.;El-Bayoumy, Karam
前列腺重要细胞通路的衰老相关变化可能促进前列腺疾病（如前列腺癌）发展风险增加的宽松环境。我们的目标是通过系统地确定生长发育和衰老对大鼠前列腺不同叶的蛋白质组学谱的影响来检查这些变化。从幼年（4个月）、成年（12个月）、老年（18个月）和高龄（24个月）不同年龄的雄性Fisher大鼠获得前列腺叶（背外侧叶，DL和腹侧叶，VL）。使用蛋白质组学方法（isobaric Tags for Relative and Absolute Quantitation, iTRAQ）鉴定各叶中不同年龄组之间的差异表达蛋白。选择DL和VL的变化通过免疫印迹分析进行验证。iTRAQ鉴定出317个高可信度的蛋白。iTRAQ在DL和VL中分别发现了12个和6个对生长发育有显著调节的蛋白，在DL和VL中分别发现了42个和29个对衰老有显著调节的蛋白。在DL和VL生长发育过程中调节的蛋白质参与多种生物过程，包括细胞通讯和发育，而在衰老过程中调节的蛋白质主要与抗氧化活性和免疫有关。免疫印迹分析证实了α-1抗胰蛋白酶、膜联蛋白A1、缺氧上调蛋白1和78 kDa葡萄糖调节蛋白的年龄相关变化。衰老导致许多前列腺蛋白和途径的变化，这些蛋白质和途径主要与炎症有关，并可能导致前列腺疾病的发展。
Aging-related changes in important cellular pathways in the prostate may promote a permissive environment for an increased risk for prostatic disease development such as prostate cancer. Our objectives were to examine for such changes, by systematically determining the effects of growth and development and aging on proteomic profiles in different lobes of the rat prostate. Prostate lobes (dorsolateral lobe, DL and ventral lobe, VL) were obtained from male Fisher rats of various ages representing young (4 months), mature (12 months), old (18 months), and very old (24 months). Differentially expressed proteins between age groups in each lobe were identified using a proteomic approach, isobaric Tags for Relative and Absolute Quantitation (iTRAQ). Select changes in the DL and VL were verified by immunoblot analysis. iTRAQ identified 317 proteins with high confidence. iTRAQ discovered 12 and 6 proteins significantly modulated in response to growth and development in the DL and VL, respectively, and 42 and 29 proteins significantly modulated in response to aging in the DL and VL, respectively. Proteins modulated during growth and development in the DL and VL are involved in a variety of biological processes including cell communication and development, whereas proteins modulated during aging were predominantly related to antioxidant activity and immunity. Immunoblot analysis verified age-related changes for α-1 antitrypsin, annexin A1, hypoxia up-regulated protein 1, and 78 kDa glucose-regulated protein. Aging results in changes in numerous prostatic proteins and pathways which are mainly linked to inflammation and may lead to prostatic disease development.
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影响因子：
11.2
作者：
Bavik, C;Coleman, I;Nelson, PS
通讯作者：
Nelson, PS
影响因子：
3.7
作者：
Bianchi-Frias, Daniella;Vakar-Lopez, Funda;Nelson, Peter S.
通讯作者：
Nelson, Peter S.
影响因子：
2.8
作者：
Hsiang, Chin-Hui;Tunoda, Toshiyuki;Ornstein, David K.
通讯作者：
Ornstein, David K.
影响因子：
2.1
作者：
An, G;Meka, CSR;Veltri, RW
通讯作者：
Veltri, RW
影响因子：
--
作者：
Fujita A;Gomes LR;Sato JR;Yamaguchi R;Thomaz CE;Sogayar MC;Miyano S
通讯作者：
Miyano S