Postoperative Complications Are Associated with Long-Term Changes in the Gut Microbiota Following Colorectal Cancer Surgery.
Postoperative Complications Are Associated with Long-Term Changes in the Gut Microbiota Following Colorectal Cancer Surgery.
复制标题
DOI：
10.3390/life11030246
复制
发表时间：
2021-03-16
期刊：
影响因子：
--
通讯作者：
Gigic B
中科院分区：
文献类型：
--
作者：
Schmitt FCF;Schneider M;Mathejczyk W;Weigand MA;Figueiredo JC;Li CI;Shibata D;Siegel EM;Toriola AT;Ulrich CM;Ulrich AB;Boutin S;Gigic B
肠道微生物群的改变已经与腹部大手术的术后并发症有关。然而，目前仍不清楚这些变化是暂时的还是长期的影响。因此，这项前瞻性临床先导性研究的目的是检查肠道微生物区系的长期变化，并将这些变化与患者的临床病程相关联。方法：应用16S-rDNA新一代测序技术对62例新诊断的结直肠癌患者的大便标本进行分析。术前、术后6、12、24个月采集粪便样本，以确定肠道微生物群，观察肠道微生物群的纵向变化。术后将患者分为有并发症组(30例)和无并发症组(32例)。有术后并发症的患者从术后6个月开始表现出明显的阿尔法多样性下降，即使在24个月后也不能缓解。有并发症的患者在6个月的随访中，微生物组的结构也比基线有了显著的变化(p=0.006)。这与肠道微生物区系中大量物种的长期减少有关，表明共生微生物区系受到影响，溃烂梭杆菌的数量长期增加。肠道微生物群的微生物组成在术后并发症患者中显示出显著的变化，可达术后24个月。
Changes in the gut microbiome have already been associated with postoperative complications in major abdominal surgery. However, it is still unclear whether these changes are transient or a long-lasting effect. Therefore, the aim of this prospective clinical pilot study was to examine long-term changes in the gut microbiota and to correlate these changes with the clinical course of the patient. Methods: In total, stool samples of 62 newly diagnosed colorectal cancer patients undergoing primary tumor resection were analyzed by 16S-rDNA next-generation sequencing. Stool samples were collected preoperatively in order to determine the gut microbiome at baseline as well as at 6, 12, and 24 months thereafter to observe longitudinal changes. Postoperatively, the study patients were separated into two groups—patients who suffered from postoperative complications (n = 30) and those without complication (n = 32). Patients with postoperative complications showed a significantly stronger reduction in the alpha diversity starting 6 months after operation, which does not resolve, even after 24 months. The structure of the microbiome was also significantly altered from baseline at six-month follow-up in patients with complications (p = 0.006). This was associated with a long-lasting decrease of a large number of species in the gut microbiota indicating an impact in the commensal microbiota and a long-lasting increase of Fusobacterium ulcerans. The microbial composition of the gut microbiome shows significant changes in patients with postoperative complications up to 24 months after surgery.
登录
查看更多内容
影响因子：
4
作者：
Matsumoto, T.;Ishikawa, H.;Yamaguchi, K.
通讯作者：
Yamaguchi, K.
影响因子：
3.1
作者：
Ojima M;Motooka D;Shimizu K;Gotoh K;Shintani A;Yoshiya K;Nakamura S;Ogura H;Iida T;Shimazu T
通讯作者：
Shimazu T
影响因子：
5.2
作者：
Derrien M;Van Baarlen P;Hooiveld G;Norin E;Müller M;de Vos WM
通讯作者：
de Vos WM
DOI：
10.1097/shk.0000000000000534
发表时间：
2016-05
期刊：
Shock (Augusta, Ga.)
影响因子：
--
作者：
Krezalek MA;DeFazio J;Zaborina O;Zaborin A;Alverdy JC
通讯作者：
Alverdy JC
影响因子：
4.8
作者：
McDonald D;Ackermann G;Khailova L;Baird C;Heyland D;Kozar R;Lemieux M;Derenski K;King J;Vis-Kampen C;Knight R;Wischmeyer PE
通讯作者：
Wischmeyer PE