Presence of a metabotropic and an ionotropic purinergic receptor on rat submandibular ductal cells.
Presence of a metabotropic and an ionotropic purinergic receptor on rat submandibular ductal cells.
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大鼠颌下导管细胞上存在代谢型和离子型嘌呤能受体。
DOI：
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发表时间：
1996
影响因子：
--
通讯作者：
J. Dehaye
中科院分区：
文献类型：
--
作者：
H. Amsallem;M. Métioui;A. Vandenabeele;A. Elyamani;A. Moran;J. Dehaye
ATP（1 μ M-1 mM）可增加大鼠下颌下腺导管细胞内Ca ~（2+）浓度（[Ca ~（2+）]i）。剂量-反应曲线是双相的，第一个平台约为10 μ M，第二个增加浓度高于100 μ M。这第二次增加被取消在细胞外钙的情况下，或在考马斯亮蓝的存在下，可以模仿苯甲酰-ATP。这种反应的激活增加了细胞外锰的摄取和导管细胞标记物激肽释放酶的释放。ADP、2-甲基硫代腺苷5 '-三磷酸（2-MeS-ATP;这是最有效的激动剂）、腺苷5'-O-（2-硫代二磷酸）和UTP模拟了低浓度ATP下的反应。去除细胞外钙没有取消这种反应，但ADP或2-MeS-ATP与毒胡萝卜素预孵育后没有效果。用2-MeS-ATP预孵育使参与对ADP和UTP的反应的受体脱敏。同样，用UTP预处理阻止了对ADP的进一步反应。ADP增加细胞内肌醇1，4，5-三磷酸的浓度，但不影响激肽释放酶的分泌。总之，大鼠下颌下腺导管细胞具有两种类型的嘌呤能受体：亲代谢型（P2 Y1）受体和亲离子型P2 X嘌呤能受体耦合到锰渗透钙通道和激肽释放酶分泌。
ATP (1 microM-1 mM) increased the intracellular Ca2+ concentration ([Ca2+]i) in rat submandibular ductal cells. The dose-response curve was biphasic with a first plateau approximately 10 microM and a second increase at concentrations higher than 100 microM. This second increase was abolished in the absence of extracellular calcium or in the presence of Coomassie blue and could be mimicked by benzoyl-ATP. The activation of this response increased the uptake of extracellular manganese and the release of kallikrein, a ductal cell marker. The response at low concentrations of ATP was mimicked by ADP, 2-methylthioadenosine 5'-triphosphate (2-MeS-ATP; which was the most potent agonist), adenosine 5'-O-(2-thiodiphosphate), and UTP. Removal of extracellular calcium did not abolish this response, but ADP or 2-MeS-ATP had no effect after a preincubation with thapsigargin. A preincubation with 2-MeS-ATP desensitized the receptor involved in the response to both ADP and UTP. Similarly, a pretreatment with UTP prevented a further response to ADP. ADP increased the intracellular concentration of inositol 1,4,5-trisphosphate but did not affect the secretion of kallikrein. In conclusion, rat submandibular ductal cells possess two types of purinergic receptors: a metabotropic (P2y1) receptor and a P2x ionotropic purinergic receptor coupled to a manganese-permeant calcium channel and to kallikrein secretion.
在大鼠腮腺腺泡细胞中，考马斯亮蓝 G 是比活性蓝 2 (Cibacron Blue 3GA) 更有效的 P2 嘌呤能反应拮抗剂。
DOI：
10.1016/0006-291x(89)92741-1
发表时间：
1989
影响因子：
3.1
作者：
Soltoff,SP;McMillian,MK;Talamo,BR
通讯作者：
Talamo,BR
通过大鼠颌下腺腺泡中的细胞外 ATP 动员 Ca2 流入，但不动员储存的 Ca2。
影响因子：
3
作者：
Hurley,TW;Ryan,MP;Shoemaker,DD
通讯作者：
Shoemaker,DD