A reconfiguration of CaV2 Ca2+ channel current and its dopaminergic D2 modulation in developing neostriatal neurons.
A reconfiguration of CaV2 Ca2+ channel current and its dopaminergic D2 modulation in developing neostriatal neurons.
复制标题
CaV2 Ca2 通道电流的重新配置及其在发育中的新纹状体神经元中的多巴胺能 D2 调节。
作者：
H. Salgado;F. Tecuapetla;T. Perez;A. Pérez‐Burgos;E. Pérez;E. Galarraga;J. Bargas
本实验研究了D(2)多巴胺受体激活对新生大鼠出生后14天和40天新生纹状体投射神经元钙电流的调制作用。D(2)类受体激动剂在两个阶段都使全细胞钙电流减少约35%，这一作用可被D(2)受体拮抗剂舒必利阻断。尼群地平在这两个阶段都部分阻断了这种调制，表明钙(V)1通道的调制存在于整个发育时期。然而，在PD40神经元中，Ca(V)1通道的调制明显更大。桔梗毒素GVIA阻断PD14神经元钙电流的大部分调制。然而，在PD40神经元中，这种闭塞明显减少。Omega-Agatoxin TK阻断了PD40神经元的大部分调制，但对PD14神经元的影响可忽略不计。数据表明，多巴胺能D(2)介导的调节在发育过程中经历了靶点的变化：从Ca(V)2.2到Ca(V)2.1 Ca(2+)通道。这种变化发生在Ca(V)2.2通道下调和Ca(V)2.1通道上调的同时。由D(2)受体介导的突触前调制反映了这些变化；幼年动物使用Ca(V)2.2型通道释放，而成熟动物很少使用，这表明这种变化同时发生在躯体树突和突触膜上。
The modulatory effect of D(2) dopamine receptor activation on calcium currents was studied in neostriatal projection neurons at two stages of rat development: postnatal day (PD)14 and PD40. D(2)-class receptor agonists reduced whole cell calcium currents by about 35% at both stages, and this effect was blocked by the D(2) receptor antagonist sulpiride. Nitrendipine partially occluded this modulation at both stages, indicating that modulation of Ca(V)1 channels was present throughout this developmental interval. Nevertheless, modulation of Ca(V)1 channels was significantly larger in PD40 neurons. omega-Conotoxin GVIA occluded most of the Ca(2+) current modulation in PD14 neurons. However, this occlusion was greatly decreased in PD40 neurons. omega-Agatoxin TK occluded a great part of the modulation in PD40 neurons but had a negligible effect in PD14 neurons. The data indicate that dopaminergic D(2)-mediated modulation undergoes a change in target during development: from Ca(V)2.2 to Ca(V)2.1 Ca(2+) channels. This change occurred while Ca(V)2.2 channels were being down-regulated and Ca(V)2.1 channels were being up-regulated. Presynaptic modulation mediated by D(2) receptors reflected these changes; Ca(V)2.2 type channels were used for release in young animals but very little in mature animals, suggesting that changes took place simultaneously at the somatodendritic and the synaptic membranes.
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影响因子：
2.5
作者：
Hu, XT;Dong, Y;White, FJ
通讯作者：
White, FJ
影响因子：
15.9
作者：
D. Surmeier;A. Reiner;M. Levine;M. A. Ariano
通讯作者：
D. Surmeier;A. Reiner;M. Levine;M. A. Ariano
DOI：
10.1016/0165-3806(90)90244-s
发表时间：
1990-03-01
期刊：
DEVELOPMENTAL BRAIN RESEARCH
影响因子：
--
作者：
BROADDUS, WC;BENNETT, JP
通讯作者：
BENNETT, JP
影响因子：
2.5
作者：
Ling, DSF;Benardo, LS
通讯作者：
Benardo, LS