Regulation of granulosa and theca cell transcriptomes during ovarian antral follicle development.
Regulation of granulosa and theca cell transcriptomes during ovarian antral follicle development.
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DOI：
10.1002/mrd.20883
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发表时间：
2008-09
影响因子：
2.5
通讯作者：
Nilsson EE
中科院分区：
文献类型：
--
作者：
Skinner MK;Schmidt M;Savenkova MI;Sadler-Riggleman I;Nilsson EE
卵巢颗粒细胞和卵泡膜细胞之间的协调相互作用是女性内分泌功能和生育能力所必需的。为了阐明这些相互作用的调控颗粒和卵泡膜细胞转录组在牛窦卵泡发育进行了研究。颗粒细胞和卵泡膜细胞分离自小（<5 mm）、中（5-10 mm）和大（>10 mm）窦牛卵泡。对24，000个牛基因的微阵列分析显示，颗粒细胞和卵泡膜细胞各自具有对小，中，大卵泡细胞特异的基因组。受管制的成绩单（即，在窦卵泡发育过程中，颗粒细胞涉及446个基因，卵泡膜细胞涉及248个基因。只有28个调节基因是共同的颗粒和卵泡膜细胞。调节基因进行功能分类，重点是两种细胞类型表达和调节的生长因子和细胞因子。介导旁分泌和自分泌细胞-细胞相互作用的候选调节生长因子蛋白包括巨噬细胞炎性蛋白（MIP 1 β）、畸胎瘤衍生生长因子1（TDGF 1）、基质衍生生长因子1（SDF 1;即，CXCL 12）、生长分化因子8（GDF 8）、神经胶质成熟因子γ（GMFG）、骨桥蛋白（SPP 1）、血管生成素4（ANGPT 4）和趋化因子配体（CCL 2、3、5和8）。本研究检测了与牛窦卵泡发育相关的颗粒细胞和卵泡膜细胞调节基因，并确定了可能参与卵巢功能所需的细胞-细胞相互作用调节的候选生长因子。
Coordinated interactions between ovarian granulosa and theca cells are required for female endocrine function and fertility. To elucidate these interactions the regulation of the granulosa and theca cell transcriptomes during bovine antral follicle development were investigated. Granulosa cells and theca cells were isolated from small (<5 mm), medium (5–10 mm), and large (>10 mm) antral bovine follicles. A microarray analysis of 24,000 bovine genes revealed that granulosa cells and theca cells each had gene sets specific to small, medium and large follicle cells. Transcripts regulated (i.e., minimally changed 1.5-fold) during antral follicle development for the granulosa cells involved 446 genes and for theca cells 248 genes. Only 28 regulated genes were common to both granulosa and theca cells. Regulated genes were functionally categorized with a focus on growth factors and cytokines expressed and regulated by the two cell types. Candidate regulatory growth factor proteins mediating both paracrine and autocrine cell–cell interactions include macrophage inflammatory protein (MIP1 beta), teratocarcinoma-derived growth factor 1 (TDGF1), stromal derived growth factor 1 (SDF1; i.e., CXCL12), growth differentiation factor 8 (GDF8), glia maturation factor gamma (GMFG), osteopontin (SPP1), angiopoietin 4 (ANGPT4), and chemokine ligands (CCL 2, 3, 5, and 8). The current study examined granulosa cell and theca cell regulated genes associated with bovine antral follicle development and identified candidate growth factors potentially involved in the regulation of cell–cell interactions required for ovarian function.
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影响因子：
2.2
作者：
Fortune, JE;Rivera, GM;Yang, MY
通讯作者：
Yang, MY
影响因子：
3.3
作者：
Bisset, LR;Schmid-Grendelmeier, P
通讯作者：
Schmid-Grendelmeier, P
影响因子：
--
作者：
Elvin, JA;Clark, AT;Matzuk, MM
通讯作者：
Matzuk, MM
DOI：
10.1016/0960-0760(93)90248-u
发表时间：
1993-03-01
影响因子：
4.1
作者：
DORRINGTON, JH;BENDELL, JJ;KHAN, SA
通讯作者：
KHAN, SA
DOI：
10.1111/j.1600-0897.1990.tb00655.x
发表时间：
1990-03-01
影响因子：
3.6
作者：
CASTILLA, JA;SAMPALO, A;HERRUZO, AJ
通讯作者：
HERRUZO, AJ