Transcript profiling of candidate genes in testis of pigs exhibiting large differences in androstenone levels.
Transcript profiling of candidate genes in testis of pigs exhibiting large differences in androstenone levels.
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DOI：
10.1186/1471-2156-11-4
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发表时间：
2010-01-25
期刊：
影响因子：
2.9
通讯作者：
Lien S
中科院分区：
文献类型：
--
作者：
Grindflek E;Berget I;Moe M;Oeth P;Lien S
野猪腐臭是一种令人不快的气味和味道的肉，发生在高比例的未阉割的雄性猪。雄甾烯酮是一种由睾丸产生的甾体激素，作为性信息素调节母猪的生殖功能，是引起公猪异味的主要物质之一。本研究的主要目的是确定与猪雄烯酮水平相关的选定候选基因的差异基因表达。本研究共纳入2560头来自挪威长白猪和杜洛克猪群的公猪。对192头脂肪中雄甾烯酮含量极高或极低的公猪睾丸样本进行RNA提取，筛选出15个候选基因，并进行实时竞争PCR分析。基因细胞色素P450 c17（CYP 17 A1）、类固醇生成急性调节蛋白（星星）、醛酮还原酶家族1成员C4（AKR 1C 4）、短链脱氢酶/还原酶家族成员4（DHRS 4）、铁蛋白轻链多肽（FTL）、磺基转移酶家族2A、脱氢表雄酮偏好成员1（SULT 2A 1）、细胞色素P450亚家族XIA多肽1（CYP 11 A1）、细胞色素b5（CYB 5A）和17-β-羟基类固醇脱氢酶IV（HSD 17 B4）在高雄甾酮的杜洛克和长白猪中均显著上调（P < 0.05）。此外，细胞色素P450 c19 A2（CYP 19 A2）下调，孕酮受体膜组分1（PGRMC 1）上调，仅在高雄甾烯酮杜洛克公猪，而CYP 21显著下调（2.5），仅在高雄甾烯酮长白猪。核受体辅激活因子4（NCOA 4）、鞘氨醇磷酸二酯酶1（SMPD 1）和3β-羟基类固醇脱氢酶（HSD 3B）基因在所有品种中的表达均无显著差异。此外，对具有一个或多个检测到的SNP的基因进行关联研究。仅在CYB 5A中观察到SNP与雄甾烯酮水平之间的关联，表明该基因的差异转录存在顺式调节。研究了一种雄甾酮水平极其极端的大型猪材料。目前的研究有助于了解哪些基因在猪中的雄甾烯酮水平方面差异表达。本文的结果表明，几个基因是重要的调节雄甾烯酮水平的公猪，值得进一步评估上述候选基因，包括在不同品种的分析，鉴定致病突变和可能的基因互作。
Boar taint is an unpleasant odor and flavor of the meat and occurs in a high proportion of uncastrated male pigs. Androstenone, a steroid produced in testis and acting as a sex pheromone regulating reproductive function in female pigs, is one of the main compounds responsible for boar taint. The primary goal of the present investigation was to determine the differential gene expression of selected candidate genes related to levels of androstenone in pigs. Altogether 2560 boars from the Norwegian Landrace and Duroc populations were included in this study. Testicle samples from the 192 boars with most extreme high or low levels of androstenone in fat were used for RNA extraction, and 15 candidate genes were selected and analyzed by real-competitive PCR analysis. The genes Cytochrome P450 c17 (CYP17A1), Steroidogenic acute regulatory protein (STAR), Aldo-keto reductase family 1 member C4 (AKR1C4), Short-chain dehydrogenase/reductase family member 4 (DHRS4), Ferritin light polypeptide (FTL), Sulfotransferase family 2A, dehydroepiandrosterone-preferring member 1 (SULT2A1), Cytochrome P450 subfamily XIA polypeptide 1 (CYP11A1), Cytochrome b5 (CYB5A), and 17-beta-Hydroxysteroid dehydrogenase IV (HSD17B4) were all found to be significantly (P < 0.05) up-regulated in high androstenone boars in both Duroc and Landrace. Furthermore, Cytochrome P450 c19A2 (CYP19A2) was down-regulated and progesterone receptor membrane component 1 (PGRMC1) was up-regulated in high-androstenone Duroc boars only, while CYP21 was significantly down-regulated (2.5) in high-androstenone Landrace only. The genes Nuclear Receptor co-activator 4 (NCOA4), Sphingomyrlin phosphodiesterase 1 (SMPD1) and 3β-hydroxysteroid dehydrogenase (HSD3B) were not significantly differentially expressed in any breeds. Additionally, association studies were performed for the genes with one or more detected SNPs. Association between SNP and androstenone level was observed in CYB5A only, suggesting cis-regulation of the differential transcription in this gene. A large pig material of highly extreme androstenone levels is investigated. The current study contributes to the knowledge about which genes that is differentially expressed regard to the levels of androstenone in pigs. Results in this paper suggest that several genes are important in the regulation of androstenone level in boars and warrant further evaluation of the above mentioned candidate genes, including analyses in different breeds, identification of causal mutations and possible gene interactions.
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影响因子：
5.1
作者：
Doran, E;Whittington, FA;McGivan, JD
通讯作者：
McGivan, JD
影响因子：
7
作者：
Lo, HS;Wang, ZN;Lee, MP
通讯作者：
Lee, MP
DOI：
10.1016/0167-4781(93)90274-h
发表时间：
1993-02-20
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
GIORDANO, SJ;STEGGLES, AW
通讯作者：
STEGGLES, AW
影响因子：
4.1
作者：
CORBIN, CJ;KHALIL, MW;CONLEY, AJ
通讯作者：
CONLEY, AJ
DOI：
10.1073/pnas.0630494100
发表时间：
2003-03-18
影响因子：
11.1
作者：
Ding, CM;Cantor, CR
通讯作者：
Cantor, CR