FLN-1/filamin is required to anchor the actomyosin cytoskeleton and for global organization of sub-cellular organelles in a contractile tissue.
FLN-1/filamin is required to anchor the actomyosin cytoskeleton and for global organization of sub-cellular organelles in a contractile tissue.
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DOI：
10.1002/cm.21633
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发表时间：
2020-10
期刊：
影响因子：
--
通讯作者：
Cram EJ
中科院分区：
文献类型：
--
作者：
Kelley CA;Triplett O;Mallick S;Burkewitz K;Mair WB;Cram EJ
肌动球蛋白网络在空间、方向、大小和连通性上组织起来，在细胞间产生协调的收缩。我们使用秀丽隐杆线虫的精囊，一个由可收缩的肌上皮细胞组成的管，来研究肌动球蛋白的结构是如何组织的。FLN-1/丝蛋白是该组织中平行、收缩、肌动球蛋白纤维形成和稳定的规则阵列所必需的。fln-1的缺失导致肌动蛋白纤维从基底表面脱离，然后沿着细胞连接处积聚并被谱蛋白稳定。此外，肌动蛋白和肌球蛋白在细胞核内被核骨架和细胞骨架复合物（LINC）的连接物捕获，形成大的病灶。细胞核的定位和形态、内质网和线粒体网络的分布也被破坏。这些结果表明，需要丝蛋白来防止大肌动蛋白束的形成和脱离，防止肌动蛋白和肌凝蛋白的过度核定位，并确保细胞器的正确定位。
Actomyosin networks are organized in space, direction, size, and connectivity to produce coordinated contractions across cells. We use the C. elegans spermatheca, a tube composed of contractile myoepithelial cells, to study how actomyosin structures are organized. FLN-1/filamin is required for the formation and stabilization of a regular array of parallel, contractile, actomyosin fibers in this tissue. Loss of fln-1 results in the detachment of actin fibers from the basal surface, which then accumulate along the cell junctions and are stabilized by spectrin. In addition, actin and myosin are captured at the nucleus by the linker of nucleoskeleton and cytoskeleton complex (LINC) complex, where they form large foci. Nuclear positioning and morphology, distribution of the endoplasmic reticulum and the mitochondrial network are also disrupted. These results demonstrate that filamin is required to prevent large actin bundle formation and detachment, to prevent excess nuclear localization of actin and myosin, and to ensure correct positioning of organelles.
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