Tissue-specific expression differences in Ras-related GTP-binding proteins in male rats.
Tissue-specific expression differences in Ras-related GTP-binding proteins in male rats.
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DOI：
10.14814/phy2.15928
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发表时间：
2024-02
影响因子：
2.5
通讯作者：
中科院分区：
文献类型：
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复合物 1 (mTORC1) 中的雷帕霉素蛋白激酶机械靶标 (mTOR) 部分受 Ras 相关 GTP 结合蛋白 (Rag GTPases) 调节。 Rag GTPases 形成异二聚体复合物，由与 RagC 或 RagD 相关的 RagA 或 RagB 组成，并起到将 mTORC1 定位到溶酶体膜的作用。直到最近，RagA 和 RagB 还被认为是功能冗余的，RagC 和 RagD 也是如此。然而，最近的研究表明，不同亚型对 mTORC1 的激活存在差异。在这里，比较了雄性大鼠骨骼肌、心脏、肝脏、肾脏和大脑中 Rag GTPases 的 mRNA 表达和蛋白质丰度。尽管在几乎所有研究的组织中，RagA 的 mRNA 表达量均高于 RagB，但在除骨骼肌之外的所有组织中，RagB 蛋白丰度均高于 RagA。在所有组织中，RagC mRNA 表达量均高于或等于 RagD mRNA，且 RagD 蛋白含量高于 RagC 蛋白。此外，肝脏中RagB的短亚型比例大于长亚型，而脑中则相反。这些结果有助于进一步阐明 Rag GTPase 的表达，并为不同组织中观察到的氨基酸差异反应提供潜在的解释。
The protein kinase Mechanistic Target of Rapamycin (mTOR) in Complex 1 (mTORC1) is regulated in part by the Ras‐related GTP‐binding proteins (Rag GTPases). Rag GTPases form a heterodimeric complex consisting of either RagA or RagB associated with either RagC or RagD and act to localize mTORC1 to the lysosomal membrane. Until recently, RagA and RagB were thought to be functionally redundant, as were RagC and RagD. However, recent research suggests that the various isoforms differentially activate mTORC1. Here, the mRNA expression and protein abundance of the Rag GTPases was compared across male rat skeletal muscle, heart, liver, kidney, and brain. Whereas mRNA expression of RagA was higher than RagB in nearly all tissues studied, RagB protein abundance was higher than RagA in all tissues besides skeletal muscle. RagC mRNA expression was more abundant or equal to RagD mRNA, and RagD protein was more abundant than RagC protein in all tissues. Moreover, the proportion of RagB in the short isoform was greater than the long in liver, whereas the opposite was true in brain. These results serve to further elucidate Rag GTPase expression and offer potential explanations for the differential responses to amino acids that are observed in different tissues.
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影响因子：
30.8
作者：
Okosun J;Wolfson RL;Wang J;Araf S;Wilkins L;Castellano BM;Escudero-Ibarz L;Al Seraihi AF;Richter J;Bernhart SH;Efeyan A;Iqbal S;Matthews J;Clear A;Guerra-Assunção JA;Bödör C;Quentmeier H;Mansbridge C;Johnson P;Davies A;Strefford JC;Packham G;Barrans S;Jack A;Du MQ;Calaminici M;Lister TA;Auer R;Montoto S;Gribben JG;Siebert R;Chelala C;Zoncu R;Sabatini DM;Fitzgibbon J
通讯作者：
Fitzgibbon J
影响因子：
21.3
作者：
通讯作者：
--
影响因子：
4.8
作者：
Meng, Delong;Yang, Qianmei;Jewell, Jenna L.
通讯作者：
Jewell, Jenna L.
DOI：
10.1126/science.1259472
发表时间：
2015-01-09
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Jewell JL;Kim YC;Russell RC;Yu FX;Park HW;Plouffe SW;Tagliabracci VS;Guan KL
通讯作者：
Guan KL
影响因子：
4.8
作者：
Bernfeld, Elyssa;Menon, Deepak;Foster, David A.
通讯作者：
Foster, David A.