Effects of Qianlie Tongqiao Capsule on Bladder Weight and Growth Factors in Bladder Tissue of Rats with Testosterone-Induced Benign Prostatic Hyperplasia
Effects of Qianlie Tongqiao Capsule on Bladder Weight and Growth Factors in Bladder Tissue of Rats with Testosterone-Induced Benign Prostatic Hyperplasia
复制标题
前列通窍胶囊对睾酮性良性前列腺增生大鼠膀胱重量及膀胱组织生长因子的影响
DOI：
10.1155/2018/5059267
复制
发表时间：
2018-11
影响因子：
--
通讯作者：
孙迪
中科院分区：
文献类型：
--
作者：
赵凡;张春和;颜俊锋;李焱风;杨毅坚;孙迪
前列通窍胶囊治疗良性前列腺增生（BPH）所致下尿路综合征及膀胱功能障碍安全有效。然而，QTC的作用机制尚不清楚。本研究旨在探讨QTC对睾酮诱导的BPH大鼠膀胱重量和膀胱组织中几种生长因子的影响。通过双侧睾丸切除术和皮下注射溶于玉米油的丙酸睾酮（5 mg/kg）建立大鼠BPH。在研究结束时，收集所有膀胱组织并称重，并使用H&E染色进行组织学检查。采用免疫组化和定量逆转录-聚合酶链反应（qRT-PCR）检测膀胱组织中神经生长因子（NGF）、碱性成纤维细胞生长因子（bFGF）和转化生长因子-β1（TGF-β1）的表达。Western Blot和qRT-PCR检测膀胱组织中Bcl-2和Bax的表达。结果表明，QTC对BPH大鼠膀胱增重及NGF、bFGF、TGF-β1的过度表达有明显的抑制作用，且以高剂量组作用最为明显。此外，QTC下调和上调前列腺梗阻后膀胱组织Bcl-2和Bax蛋白和mRNA的表达。QTC还能平衡Bcl-2/Bax比值。总体而言，这些结果揭示了QTC治疗前列腺增生引起的膀胱功能障碍的可能作用机制，还需要进一步研究。
Qianlie Tongqiao Capsule (QTC) is clinically confirmed to be efficacious and safe in treating lower urinary tract syndromes and bladder dysfunction that are induced by benign prostatic hyperplasia (BPH). However, the functional mechanisms of QTC remain unclear. We aim to investigate the effects of QTC on both bladder weight and several growth factors in the bladder tissue of rats with testosterone-induced BPH. BPH in the rats was established through bilateral orchiectomy and subcutaneous administration of testosterone propionate (5 mg/kg) dissolved in corn oil. At the end of the study, all bladder tissues were collected and weighed, and a histological examination was conducted using H&E staining. Immunohistochemistry and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) were applied to detect the expression of nerve growth factor (NGF), basic fibroblast growth factor (bFGF), and transformation growth factor-β1 (TGF-β1) in the bladder tissue. The expression of Bcl-2 and Bax in the bladder tissue was tested by Western Blot and qRT-PCR. We found that QTC, especially when administered in high-dosages, had a significant inhibitory effect on bladder weight gain and overexpression of NGF, bFGF, and TGF-β1 in rats with BPH. In addition, QTC downregulated and upregulated protein and mRNA expression of Bcl-2 and Bax in the bladder after prostatic obstruction, respectively. Furthermore, QTC balanced the Bcl-2/Bax ratio. Overall, these results reveal possible functional mechanisms of QTC in treating BPH-caused bladder dysfunction, and further studies are needed.
登录
查看更多内容
SQW对缺乏TRPV1小鼠的膀胱功能的影响。
影响因子：
--
作者：
Lai H;Yan Q;Cao H;Chen P;Xu Y;Jiang W;Wu Q;Huang P;Tan B
通讯作者：
Tan B
DOI：
10.1016/b978-0-323-43044-9.00154-0
发表时间：
2020
期刊：
Textbook of Natural Medicine
影响因子：
--
作者：
G. Espinosa;Ralph Esposito
通讯作者：
G. Espinosa;Ralph Esposito
影响因子：
3
作者：
M. Khan;Nileema Shukla;C. Thompson;F. Mumtaz;D. P. Mikhailidis;R. J. Morgan
通讯作者：
M. Khan;Nileema Shukla;C. Thompson;F. Mumtaz;D. P. Mikhailidis;R. J. Morgan
影响因子：
6.6
作者：
Deveaud, CM;Macarak, EJ;Howard, PS
通讯作者：
Howard, PS
影响因子：
2.5
作者：
A. Tubaro;S. Mariani;C. de Nunzio;R. Miano
通讯作者：
A. Tubaro;S. Mariani;C. de Nunzio;R. Miano