Effect of SQW on the bladder function of mice lacking TRPV1.
Effect of SQW on the bladder function of mice lacking TRPV1.
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SQW对缺乏TRPV1小鼠的膀胱功能的影响。
DOI：
10.1186/s12906-016-1420-6
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发表时间：
2016-11-15
影响因子：
--
通讯作者：
Tan B
中科院分区：
文献类型：
--
作者：
Lai H;Yan Q;Cao H;Chen P;Xu Y;Jiang W;Wu Q;Huang P;Tan B
缩泉丸是治疗下尿路症状（LUTS）的有效中药方剂，对膀胱过度活动症大鼠膀胱功能恢复过程中瞬时受体电位香草酸1（TRPV 1）的表达具有调节作用。本研究利用TRPV 1基因敲除（KO）小鼠进一步研究SQW调节TRPV 1信号传导和膀胱功能的机制。使用野生型和TRPV 1 KO小鼠进行研究。KO动物分为KO组和SQW治疗组。我们应用体内膀胱测压记录技术来分析膀胱的排尿控制，以及体外器官浴来研究膀胱对各种化合物的扩张反应，这些化合物随后引起正常平滑肌兴奋。实时聚合酶链反应和蛋白质印迹分析进行定量TRPV 1和P2 X3在膀胱中的表达。使用荧光素酶ATP生物发光测定试剂盒测量从膀胱条释放的ATP。KO制剂可抑制排尿次数减少，而增加排尿间隔和排尿量。在NS输注期间TRPV 1 −/−小鼠的尿动力学记录结果显示膀胱压力和收缩降低，表现出对α，β-me ATP、KCl和卡巴胆碱的反应降低，对CAP无反应。TRPV 1 −/−小鼠从膀胱平滑肌条释放的ATP显著减少，沿着无TRPV 1表达和膀胱中P2 X3表达水平降低。SQW可在一定程度上增加ATP的释放，但对TRPV 1和P2 X3的表达无影响。SQW对α，β-meATP、CAP、卡巴胆碱梯度浓度、KCl的力反应、MBC和排尿活动无明显影响。TRPV 1在膀胱机械敏感性中起重要作用。有效的SQW对无TRPV 1的小鼠膀胱功能难以发挥应有的作用。本文的在线版本（doi：10.1186/s12906-016-1420-6）包含补充材料，可供授权用户使用。
Suo Quan Wan (SQW) is an effective traditional Chinese prescription on treated lower urinary tract symptoms (LUTS), and has been proved have modulation effect on the expression of transient receptor potential vanilloid 1 (TRPV1) in accordance with the recovery of bladder function of overactive bladder rat. This study further investigated the mechanism of SQW modulated TRPV1 signaling and bladder function using TRPV1 knockout (KO) mice. Study was conducted using wild type and TRPV1 KO mice. The KO animals were grouped into KO group and SQW treated group. We applied in vivo cystometrogram recording techniques to analyze voiding control of the urinary bladder, as well as in vitro organ bath to study bladder distension response to various compounds, which subsequently elicited normal smooth muscle excitation. Real-time polymerase chain reaction and western blot analysis were performed to quantify the expression of TRPV1 and P2X3 in the bladder. ATP released from bladder strips was measured using the luciferin–luciferase ATP bioluminescence assay kit. KO preparation inhibited decrease micturition times, while micturition interval and volume were increased. Results of urodynamic record of the TRPV1−/− mice during NS infusion showed reduced bladder pressure and contraction which exhibited decreased response to α, β-me ATP, KCl, and carbachol and no response to CAP. The ATP released by the TRPV1−/− mice from strips of bladder smooth muscles was significantly reduced, along with no TRPV1 expression and reduced expression level of P2X3 in the bladder. SQW could increase ATP release in some degree, while had no effect on TRPV1 and P2X3 expression. SQW could improve bladder pressure slightly, while make no significantly effects on the force response to α,β-meATP, CAP, carbachol in gradient concentration, and KCl, as well as MBC and voiding activities. TRPV1 plays an important role in urinary bladder mechanosensitivity. The effective SQW is hard to play its proper role on bladder function of mice without TRPV1. The online version of this article (doi:10.1186/s12906-016-1420-6) contains supplementary material, which is available to authorized users.
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影响因子：
5.4
作者：
Choi, Bo-Hwa;Jin, Long-Hu;Lee, Tack
通讯作者：
Lee, Tack
影响因子：
4.5
作者：
Mattiasson, A;Blaakaer, J;Wein, AJ
通讯作者：
Wein, AJ
影响因子：
6
作者：
Brito R;Sheth S;Mukherjea D;Rybak LP;Ramkumar V
通讯作者：
Ramkumar V
中药锁泉丸对膀胱出口梗阻大鼠膀胱TRPV1表达的影响
影响因子：
--
作者：
Lai H;Tan B;Liang Z;Yan Q;Lian Q;Wu Q;Huang P;Cao H
通讯作者：
Cao H
影响因子：
2
作者：
Gevaert, Thomas;Vandepitte, Joachim;De Ridder, Dirk
通讯作者：
De Ridder, Dirk