3D and 4D imaging of immune cells in vitro and in vivo

3D and 4D imaging of immune cells in vitro and in vivo
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体外和体内免疫细胞的 3D 和 4D 成像

DOI:
10.1007/s00418-008-0520-x
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发表时间:
2008
影响因子:
2.3
通讯作者:
M. Gunzer.
M. Gunzer.
中科院分区:
生物学3区
文献类型:
--
作者:
C. Nitschke;A. Garin;M. Kosco-Vilbois;M. Gunzer.

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分析细胞免疫反应的动态,虽然在免疫学研究中进行了几十年,但自从活体双光子显微镜的发展以来,使用这种方法的研究数量大幅增加。与此同时,对细胞免疫动力学的新见解每天都在发表。这篇评论给出了一个简短的概述,目前最广泛使用的技术,无论是在显微镜方面,以及在实验部分。困难和承诺将被讨论。最后,一个人的选择,最有趣的发现,前6年的活体2-光子显微镜免疫学问题将给出。总的目的是让读者对这种通过“动态组织学”研究免疫反应的迷人方式感兴趣。这已经并将继续拓宽我们对免疫细胞在真实的生活中如何工作的看法。
Analyzing the dynamics of cellular immune responses, although performed for decades in immunologic research, has seen an enormous increase in the number of studies using this approach since the development of intravital 2-photon microscopy. Meanwhile, new insights into the dynamics of cellular immunity are being published on a daily basis. This review gives a short overview of the currently most widely used techniques, both on the microscopy side as well as on the experimental part. Difficulties and promises will be discussed. Finally, a personal selection of the most interesting findings of the first 6 years of intravital 2-photon microscopy for immunological questions will be given. The overall aim is to get the reader interested into this fascinating way of investigating the immune response by means of “dynamic histology”. This already has and will continue to broaden our view on how immune cells work in real life.
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影响因子: 1.1
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