Low intraprostatic DHT promotes the infiltration of CD8+ T cells in BPH tissues via modulation of CCL5 secretion.
Low intraprostatic DHT promotes the infiltration of CD8+ T cells in BPH tissues via modulation of CCL5 secretion.
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低前列腺内 DHT 通过调节 CCL5 分泌促进 BPH 组织中 CD8 T 细胞的浸润
DOI：
10.1155/2014/397815
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发表时间：
2014
影响因子：
4.6
通讯作者：
Jin J
中科院分区：
文献类型：
--
作者：
Fan Y;Hu S;Liu J;Xiao F;Li X;Yu W;Cui Y;Sun M;Lv T;He Q;Jin J
临床研究表明，雄激素可能与良性前列腺增生症(BPH)患者前列腺内T细胞的浸润有关，但T细胞亚群及其作用机制尚不清楚。本研究验证了前列腺内注射5-二氢睾酮(5-α-diwater testerone，DHT)对前列腺上皮细胞T细胞募集的影响。将行经尿道前列腺电切术(TURP)后的前列腺组织分为两组：(1)无用药史组，(2)术前给予5-α-还原酶II型抑制剂非那雄胺5 mg/d，至少6个月。免疫组织化学和流式细胞仪检测显示，2组CD8+T细胞的浸润率明显高于1组，而CD4+T细胞的比例无明显变化(P>0.05)。在体外实验中，在低DHT条件下，组2和BPH-1细胞的CD8+T细胞向前列腺组织裂解液中迁移较多。在低DHT条件下，BPH-1细胞趋化因子(C-C基序)配体5(CCL5)和CD8+T细胞趋化因子(C-C基序)受体5(CCR5)mRNA转录上调。免疫组织化学检测发现，CCL5在第2组前列腺组织中的表达也较高。本研究提示，前列腺腔内DHT可能通过调节CCL5的分泌，参与调节人前列腺上皮细胞的炎症反应。
Clinical studies suggested thatandrogen might be associated with infiltrating T cells in prostate of benign prostatic hyperplasia (BPH) patients, but detail of T-cell subset and mechanism still remained unclear. The present study tested the hypothesis that intraprostatic 5α-dihydrotestosterone (DHT) exerts effects on T cells recruitment by BPH epithelial cells. Prostate tissues from 64 cases of BPH patients after transurethral resection of prostate (TURP) were divided into 2 groups: (1) no medication history; (2) administration of 5α-reductase type II inhibitor-finasteride 5 mg daily for at least 6 months before surgery. Group 2 presented significantly higher CD8+ T cells infiltration than group 1, but no changes in CD4+ T cells (immunohistochemistry and flow cytometry). In vitro study more CD8+ T cell migrated to the prostate tissue lysates from group 2 and BPH-1 cells in low DHT condition. Transcription of chemokine (C-C motif) Ligand 5 (CCL5) mRNA in BPH-1 cells and chemokine (C-C motif) receptor 5 (CCR5) mRNA in CD8+ T cells were upregulated in low DHT condition (q-PCR). CCL5 expression was also identified to be higher in group 2 prostate tissues by IHC. This study suggested that intraprostatic DHT may participate in regulating inflammatory response which was induced by human prostatic epithelial cell, via modulating CCL5 secretion.
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影响因子：
4
作者：
Vignozzi, Linda;Cellai, Ilaria;Maggi, Mario
通讯作者：
Maggi, Mario
影响因子：
4
作者：
Vignozzi, Linda;Morelli, Annamaria;Maggi, Mario
通讯作者：
Maggi, Mario
影响因子：
23.4
作者：
Nickel, J. Curtis;Roehrborn, Claus G.;Rittmaster, Roger S.
通讯作者：
Rittmaster, Roger S.
影响因子：
6.6
作者：
Wei, JT;Calhoun, E;Jacobsen, SJ
通讯作者：
Jacobsen, SJ
DOI：
10.1073/pnas.89.22.10787
发表时间：
1992-11-15
影响因子：
11.1
作者：
HARRIS, G;AZZOLINA, B;ELLSWORTH, K
通讯作者：
ELLSWORTH, K