Stable small quantum dots for synaptic receptor tracking on live neurons.

Stable small quantum dots for synaptic receptor tracking on live neurons.
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DOI:
10.1002/anie.201405735
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发表时间:
2014-11-10
影响因子:
16.6
通讯作者:
Selvin, Paul R.
Selvin, Paul R.
中科院分区:
化学1区
文献类型:
--
作者:
Cai, En;Ge, Pinghua;Lee, Sang Hak;Jeyifous, Okunola;Wang, Yong;Liu, Yanxin;Wilson, Katie M.;Lim, Sung Jun;Baird, Michelle A.;Stone, John E.;Lee, Kwan Young;Davidson, Michael W.;Chung, Hee Jung;Schulten, Klaus;Smith, Andrew M.;Green, William N.;Selvin, Paul R.

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We developed a coating method to produce functionalized small quantum dots (sQDs), ~ 9 nm in diameter, that were stable for over a month. We made sQDs in four emission wavelengths, from 527 nm to 655 nm and with different functional groups. AMPA receptors on live neurons were labeled with sQDs and post-synaptic density proteins were visualized with super-resolution microscopy. Their diffusion behavior indicates that sQDs access the synaptic clefts significantly more often than commercial QDs.
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