OM Mutations in DROSOPHILA ANANASSAE Are Linked to Insertions of a Transposable Element.
OM Mutations in DROSOPHILA ANANASSAE Are Linked to Insertions of a Transposable Element.
复制标题
果蝇 ANANASSAE 中的 OM 突变与转座元件的插入有关。
作者：
A. Shrimpton;E. Montgomery;C. Langley
据推测，果蝇的Om突变（涉及20个位点的自发突变，导致半显性、非多效性的眼睛形态缺陷）是由于转座元件tom的插入引起的。一个特别不稳定的x连锁Om等位基因产生了几个衍生物，其中一个具有更极端的Om表型，并伴随着一个烧焦的刚毛突变，sn（9g）。利用黑腹龙sn位点DNA探针克隆了黑腹龙的同源区。分析sn(9g) DNA检测到6.5 kb的插入。基因组Southern印迹和原位杂交技术表明，该插入物是重复和分散的。遗传作图证实了tom元件的存在，在被测的4个x连锁位点上，6.5 kb的sn（9g）插入体和Om突变体之间建立了同源性。
It has been hypothesized that Om mutability in Drosophila ananassae (involving spontaneous mutation at 20 loci, resulting in semidominant, nonpleiotropic eye morphology defects) was due to insertion of a transposable element, tom. One particularly unstable X-linked Om allele produced several derivatives, one of which has a more extreme Om phenotype and was accompanied by a singed bristle mutant, sn( 9g). DNA probes from the sn locus of D. melanogaster were used to clone the homologous region of D. ananassae. Analysis of sn(9g) DNA detected a 6.5-kb insert. Genomic Southern blotting and in situ hybridization techniques showed that this insert is repetitive and dispersed. The existence of the tom element is supported by genetic mapping that established homology between the 6.5-kb sn(9g) insert and Om mutants at the four X-linked loci tested.
果蝇突变抑制系统中的核细胞质关系。
影响因子：
3.3
作者：
Hinton,CW
通讯作者：
Hinton,CW
DOI：
10.1073/pnas.78.11.6633
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
LANGER, PR;WALDROP, AA;WARD, DC
通讯作者：
WARD, DC
DOI：
10.1073/pnas.79.14.4381
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
LANGERSAFER, PR;LEVINE, M;WARD, DC
通讯作者：
WARD, DC