Prevention of antibiotic-associated metabolic syndrome in mice by intestinal alkaline phosphatase.
Prevention of antibiotic-associated metabolic syndrome in mice by intestinal alkaline phosphatase.
复制标题
通过肠道碱性磷酸酶预防小鼠中与抗生素相关的代谢综合征。
DOI：
10.1111/dom.12645
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发表时间：
2016-05
期刊：
影响因子：
--
通讯作者：
Hodin RA
中科院分区：
文献类型：
--
作者：
Economopoulos KP;Ward NL;Phillips CD;Teshager A;Patel P;Mohamed MM;Hakimian S;Cox SB;Ahmed R;Moaven O;Kaliannan K;Alam SN;Haller JF;Goldstein AM;Bhan AK;Malo MS;Hodin RA
儿童早期接触抗生素与成年后代谢综合征（MetS）的发病机制有关。肠道碱性磷酸酶（IAP）可维持肠道微生物群的正常稳态，并在抗生素治疗停止后恢复正常微生物群。我们的目的是研究生命早期联合使用 IAP 与抗生素是否可以对小鼠 MetS 具有预防作用。断奶后将五十只小鼠分配至四个治疗组。小鼠接受阿奇霉素±IAP治疗，或不接受阿奇霉素±IAP治疗，持续三个间歇的7天周期。最后一个疗程后，对小鼠进行五周的常规饮食，随后进行五周的高脂肪饮食。比较动物体重、食物摄入量、水摄入量、血脂、葡萄糖水平和肝脂。 16S rRNA 基因焦磷酸测序用于确定微生物组组成的差异。生命早期接触阿奇霉素使小鼠在成年后易患 MetS。 IAP 与阿奇霉素联合给药可将总体重、血脂、葡萄糖水平和肝脂降低至对照小鼠的水平，从而完全防止了这种敏感性。 IAP 的这些影响可能是由于属和种水平上特定细菌类群（例如厌氧原体和副拟杆菌属的成员）的组成发生变化而发生的。在生命早期同时给予 IAP 和阿奇霉素可防止小鼠对后期 MetS 的易感性。这种效应与肠道微生物群组成的改变有关。 IAP 可能代表一种针对人类 MetS 的新疗法。
Early childhood exposure to antibiotics has been implicated in the pathogenesis of metabolic syndrome (MetS) later on in adulthood. Intestinal alkaline phosphatase (IAP) preserves the normal homeostasis of intestinal microbiota and restores the normal microbiota upon cessation of antibiotic treatment. We aim to examine whether co-administration of IAP with antibiotics early in life may have a preventive role against MetS in mice. Fifty mice were allocated to four treatment groups after weaning. Mice were treated with azithromycin±IAP, or with no azithromycin±IAP, for three intermittent 7-day cycles. After the last treatment course, the mice were administered regular chow diet for five weeks and subsequently high-fat diet for five weeks. Animal body weight, food intake, water intake, serum lipids, glucose levels and liver lipids were compared. 16S rRNA gene pyrosequencing was used to determine differences in microbiome composition. Azithromycin exposure early in life rendered mice susceptible to MetS in adulthood. Co-administration of IAP with azithromycin completely prevented this susceptibility by decreasing total body weight, serum lipids, glucose levels and liver lipids to the levels of control mice. These effects of IAP likely occur due to changes in the composition of specific bacterial taxa at the genus and species levels (e.g. members of Anaeroplasma and Parabacteroides). Co-administration of IAP with azithromycin early in life prevents mice from susceptibility to the later development of MetS. This effect is associated with alterations in the composition of the gut microbiota. IAP may represent a novel treatment against MetS in humans.
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DOI：
10.1038/ismej.2014.45
发表时间：
2014-10
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
30.3
作者：
Bates, Jennifer M.;Akerlund, Janie;Guillemin, Karen
通讯作者：
Guillemin, Karen
影响因子：
8
作者：
Hersh, Adam L.;Shapiro, Daniel J.;Shah, Samir S.
通讯作者：
Shah, Samir S.
影响因子：
4.9
作者：
Ajslev, T. A.;Andersen, C. S.;Jess, T.
通讯作者：
Jess, T.
影响因子：
64.5
作者：
Cox LM;Yamanishi S;Sohn J;Alekseyenko AV;Leung JM;Cho I;Kim SG;Li H;Gao Z;Mahana D;Zárate Rodriguez JG;Rogers AB;Robine N;Loke P;Blaser MJ
通讯作者：
Blaser MJ