Separating the spindle, checkpoint, and timer functions of BubR1.
Separating the spindle, checkpoint, and timer functions of BubR1.
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DOI：
10.1083/jcb.200905026
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发表时间：
2009-11-30
期刊：
影响因子：
--
通讯作者：
Karess RE
中科院分区：
文献类型：
--
作者：
Rahmani Z;Gagou ME;Lefebvre C;Emre D;Karess RE
The BubR1 kinase domain controls spindle attachment to the kinetochores, whereas the KEN domain regulates activation of the spindle assembly checkpoint. BubR1 performs several roles during mitosis, affecting the spindle assembly checkpoint (SAC), mitotic timing, and spindle function, but the interdependence of these functions is unclear. We have analyzed in Drosophila melanogaster the mitotic phenotypes of kinase-dead (KD) BubR1 and BubR1 lacking the N-terminal KEN box. bubR1-KD individuals have a robust SAC but abnormal spindles with thin kinetochore fibers, suggesting that the kinase activity modulates microtubule capture and/or dynamics but is relatively dispensable for SAC function. In contrast, bubR1-KEN flies have normal spindles but no SAC. Nevertheless, mitotic timing is normal as long as Mad2 is present. Thus, the SAC, timer, and spindle functions of BubR1 are substantially separable. Timing is shorter in bubR1-KEN mad2 double mutants, yet in these flies, lacking both critical SAC components, chromosomes still segregate accurately, reconfirming that in Drosophila, reliable mitosis does not need the SAC.
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影响因子：
11.8
作者：
Malureanu, Liviu A.;Jeganathan, Karthik B.;Hamada, Masakazu;Wasilewski, Lisa;Davenport, James;van Deursen, Jan M.
通讯作者：
van Deursen, Jan M.
影响因子：
21.3
作者：
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通讯作者：
Pines, Jonathon
影响因子：
3.7
作者：
Harris, L;Davenport, J;Goorha, R
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影响因子：
21.3
作者：
Basto, R;Gomes, R;Karess, RE
通讯作者：
Karess, RE
DOI：
10.1073/pnas.081076898
发表时间：
2001-04-10
影响因子：
11.1
作者：
Skoufias, DA;Andreassen, PR;Margolis, RL
通讯作者：
Margolis, RL