The canine oral microbiome.
The canine oral microbiome.
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犬口腔微生物组。
DOI：
10.1371/journal.pone.0036067
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Marshall-Jones ZV
中科院分区：
文献类型：
--
作者：
Dewhirst FE;Klein EA;Thompson EC;Blanton JM;Chen T;Milella L;Buckley CM;Davis IJ;Bennett ML;Marshall-Jones ZV
确定犬口腔微生物组的细菌组成有两个主要原因。首先，虽然人类口腔微生物组已经使用分子技术进行了很好的研究，但其他哺乳动物的口腔微生物组尚未使用独立于培养物的方法进行同等深入的研究。这项研究允许细菌分类群的数量进行比较，基于16S rRNA基因序列比较，人类和狗，两个不同的哺乳动物物种之间共享。其次，犬口腔细菌是兽医和人类医学界感兴趣的，以了解它们在健康和传染病中的作用。所涉及的细菌大多未命名，并且未通过16S rRNA基因序列同一性与分类学方案相关联。本文描述了对来自65个克隆文库的5，958个16S rRNA基因序列的分析。获得了353个犬细菌分类群的16SrRNA参考序列，分属于14个细菌门、23个纲、37个目、66个科、148个属。80%的分类群目前还没有命名。在狗中鉴定的细菌分类群与人类的那些明显不同，基于98.5%的16S rRNA序列相似性截止值，狗和人类之间只有16.4%的口腔分类群是共享的。这表明，在构成不同哺乳动物物种的口腔微生物组的细菌中存在很大的差异。基于与人类细菌的表型相似性来鉴定动物相关细菌的历史实践通常是无效的。本报告描述了犬口腔微生物组的多样性，并提供了一个临时的基于16S rRNA的分类方案，用于命名和鉴定未命名的犬细菌分类群。
Determining the bacterial composition of the canine oral microbiome is of interest for two primary reasons. First, while the human oral microbiome has been well studied using molecular techniques, the oral microbiomes of other mammals have not been studied in equal depth using culture independent methods. This study allows a comparison of the number of bacterial taxa, based on 16S rRNA-gene sequence comparison, shared between humans and dogs, two divergent mammalian species. Second, canine oral bacteria are of interest to veterinary and human medical communities for understanding their roles in health and infectious diseases. The bacteria involved are mostly unnamed and not linked by 16S rRNA-gene sequence identity to a taxonomic scheme. This manuscript describes the analysis of 5,958 16S rRNA-gene sequences from 65 clone libraries. Full length 16S rRNA reference sequences have been obtained for 353 canine bacterial taxa, which were placed in 14 bacterial phyla, 23 classes, 37 orders, 66 families, and 148 genera. Eighty percent of the taxa are currently unnamed. The bacterial taxa identified in dogs are markedly different from those of humans with only 16.4% of oral taxa are shared between dogs and humans based on a 98.5% 16S rRNA sequence similarity cutoff. This indicates that there is a large divergence in the bacteria comprising the oral microbiomes of divergent mammalian species. The historic practice of identifying animal associated bacteria based on phenotypic similarities to human bacteria is generally invalid. This report describes the diversity of the canine oral microbiome and provides a provisional 16S rRNA based taxonomic scheme for naming and identifying unnamed canine bacterial taxa.
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影响因子：
4.4
作者：
Ashelford, Kevin E.;Chuzhanova, Nadia A.;Weightman, Andrew J.
通讯作者：
Weightman, Andrew J.
DOI：
10.1099/00207713-44-4-812
发表时间：
1994-10-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
COLLINS, MD;LAWSON, PA;FARROW, JAE
通讯作者：
FARROW, JAE
影响因子：
2.2
作者：
Allaker, RP;deRosayro, R;Hardie, JM
通讯作者：
Hardie, JM
影响因子：
3.3
作者：
Hardham, J;Dreier, K;Evans, RT
通讯作者：
Evans, RT
DOI：
10.1099/ijs.0.01934-0
发表时间：
2002-07-01
影响因子：
2.8
作者：
Hoyles, L;Falsen, E;Collins, MD
通讯作者：
Collins, MD