Enhancement of DNA synthesis and cell proliferation by 1-methylnicotinamide in rat liver cells in culture: implication for its in vivo role.
Enhancement of DNA synthesis and cell proliferation by 1-methylnicotinamide in rat liver cells in culture: implication for its in vivo role.
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1-甲基烟酰胺在培养的大鼠肝细胞中增强 DNA 合成和细胞增殖：对其体内作用的影响。
DOI：
10.1016/0006-291x(82)90950-0
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发表时间：
1982
影响因子：
3.1
通讯作者：
H. Kröger
中科院分区：
文献类型：
--
作者：
J. Hoshino;U. Kühne;H. Kröger
1-甲基烟酰胺是烟酰胺的直接甲基化产物，在高于20 μM的浓度下刺激培养的大鼠肝细胞（RLC）的DNA合成和增殖。烟酰胺是一种有效的DNA合成和增殖抑制剂，其作用可被1-甲基烟酰胺抵消。在1-甲基烟酰胺下，细胞内NAD浓度在2小时内降低，而在烟酰胺的存在下增加。在分离的核中的聚（ADP-核糖）合成活性保持不变。这些结果表明1-甲基烟酰胺通过降低细胞内NAD水平在细胞生长中的生理作用。
1-Methylnicotinamide, a direct methylation product of nicotinamide, stimulates the DNA synthesis and proliferation of rat liver cells (RLC) in culture at concentrations higher than 20 μM. The effect of nicotinamide, which is a potent inhibitor of DNA synthesis and proliferation, is counteracted by 1-methylnicotinamide. The intracellular NAD concentration decreases within 2 h under 1-methylnicotinamide, whereas it increases in the presence of nicotinamide. The poly(ADP-ribose) synthesizing activity in the isolated nuclei remained unchanged. These results suggest a physiological role of 1-methylnicotinamide in the cell growth through a lowering of intracellular NAD level.
烟酰胺对培养肝细胞中非计划 DNA 合成的影响。
DOI：
10.1016/0006-291x(80)91581-8
发表时间：
1980
影响因子：
3.1
作者：
Althaus,FR;Lawrence,SD;Sattler,GL;Pitot,HC
通讯作者：
Pitot,HC