Using en-face optical coherence tomography to analyse gene function in Drosophila Melanogaster larval heart
Using en-face optical coherence tomography to analyse gene function in Drosophila Melanogaster larval heart
复制标题
使用正面光学相干断层扫描分析果蝇幼虫心脏的基因功能
DOI：
10.1117/12.814932
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Bradu A
中科院分区：
文献类型：
--
作者：
Bradu A
果蝇幼虫心脏的体内光学相干断层扫描（OCT）成像允许无创可视化和评估其心脏功能。为了对果蝇心脏进行成像，我们开发了一种能够同时提供OCT和激光扫描共聚焦显微镜（LSCM）图像的专用成像仪器。通过这种双成像系统，可以很容易地在样本中定位和可视化心脏，并监测心动周期中心脏形状的变化。在这里，我们已经使用靶向基因表达敲低myospheroid（mys）基因在幼虫心脏使用特定的RNAi构建。通过敲除由mys编码的β整合素亚单位，我们记录到舒张和收缩状态下的扩大的心腔。此外，在敲除心脏中，腔室直径减小的分数较小。这些表型差异表明心脏收缩力受损发生在心脏中，其中整合素基因表达水平降低。据我们所知，这是第一次表明整合素与扩张性心脏缺陷有直接关系。
In-vivoOptical Coherence Tomography (OCT) imaging of the fruit flyDrosophila Melanogasterlarval heart allows non invasive visualizations and assessment of its cardiac function. In order to image Drosophila heart, we have developed a dedicated imaging instrument able to provide simultaneous OCT and Laser Scanning Confocal Microscopy (LSCM) images. With this dual imaging system, the heart can easily be located and visualised within the specimen and the change of the heart shape in a cardiac cycle monitored. Here we have used targeted gene expression to knockdown the myospheroid (mys) gene in the larval heart using a specific RNAi construct. By knocking down a β integrin subunit encoded by mys we have recorded an enlarged heart chamber in both diastolic and systolic states. Also, the fraction of reduction of the chamber diameter was smaller in the knockdown heart. These phenotypic differences indicate that impaired cardiac contractility occurs in the heart where the integrin gene express level is reduced. At our knowledge, this is for the first time when it is shown that integrins have a direct relationship to a dilated heart defect.
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影响因子：
20.1
作者：
Paternostro, G;Vignola, C;Reed, JC
通讯作者：
Reed, JC
奥斯蒂亚
影响因子：
0.1
作者：
W. Rollo
通讯作者：
W. Rollo
影响因子：
3.6
作者：
Podoleanu, AG;Dobre, GM;Jackson, DA
通讯作者：
Jackson, DA
影响因子：
3.6
作者：
Podoleanu, AG;Dobre, GM;Jackson, DA
通讯作者：
Jackson, DA