The Intestinal Microbiome Predicts Weight Loss on a Calorie-Restricted Diet and Is Associated With Improved Hepatic Steatosis.
The Intestinal Microbiome Predicts Weight Loss on a Calorie-Restricted Diet and Is Associated With Improved Hepatic Steatosis.
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DOI：
10.3389/fnut.2021.718661
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发表时间：
2021
影响因子：
5
通讯作者：
Jacobs JP
中科院分区：
文献类型：
--
作者：
Dong TS;Luu K;Lagishetty V;Sedighian F;Woo SL;Dreskin BW;Katzka W;Chang C;Zhou Y;Arias-Jayo N;Yang J;Ahdoot AI;Ye J;Li Z;Pisegna JR;Jacobs JP
背景资料：在临床前和流行病学研究中，微生物组已被证明在肥胖和代谢相关性脂肪肝（MAFLD）的发展和治疗中都很重要。然而，很少有研究探讨微生物组在卡路里限制的临床反应中的作用。为了探索这一领域，我们进行了一项前瞻性研究，研究肠道微生物组与热量限制饮食中体重减轻和肝脂肪变性变化的关系。研究方法：在大西部洛杉矶退伍军人事务医院对80名超重和肥胖的参与者进行了一项前瞻性饮食干预研究。患者接受16周的大量营养素标准化饮食，包括14周的卡路里限制（500卡路里不足）。在基线和第16周时，通过阻抗、血浆脂质测量和超声弹性成像测量肝脏脂肪变性进行身体成分分析。使用16S rRNA基因测序评估肠道微生物组组成。对完成试验的所有受试者（n = 46）进行符合方案分析。结果：研究完成者的体重、体重指数、总胆固醇、低密度脂蛋白和甘油三酯显著降低。体重减轻至少5%的受试者比体重减轻<5%的受试者血清甘油三酯和肝脏脂肪变性的降低幅度显着更大。试验结束时肠球菌和克雷伯菌减少，而粪球菌和Collinsella增加。体重减轻至少5%的患者与体重未减轻的患者之间也存在显著的基线微生物组差异。毛梭菌与肝脂肪变性呈正相关，放线菌与肝脂肪变性和体重呈正相关。基线微生物组特征能够预测哪些患者体重减轻至少5%，AUROC为0.80。结论：热量限制改变了肠道微生物组，并改善了那些体重显著减轻的人的肝脏脂肪变性。基线微生物组差异预测热量限制饮食的体重减轻，并与肝脏脂肪变性的改善相关，表明肠道微生物组在介导热量限制的临床反应中的作用。
Background: The microbiome has been shown in pre-clinical and epidemiological studies to be important in both the development and treatment of obesity and metabolic associated fatty liver disease (MAFLD). However, few studies have examined the role of the microbiome in the clinical response to calorie restriction. To explore this area, we performed a prospective study examining the association of the intestinal microbiome with weight loss and change in hepatic steatosis on a calorie-restricted diet. Methods: A prospective dietary intervention study of 80 overweight and obese participants was performed at the Greater West Los Angeles Veterans Affair Hospital. Patients were placed on a macronutrient standardized diet for 16 weeks, including 14 weeks of calorie restriction (500 calorie deficit). Body composition analysis by impedance, plasma lipid measurements, and ultrasound elastography to measure hepatic steatosis were performed at baseline and week 16. Intestinal microbiome composition was assessed using 16S rRNA gene sequencing. A per protocol analysis was performed on all subjects completing the trial (n = 46). Results: Study completers showed significant reduction in weight, body mass index, total cholesterol, low density lipoprotein, and triglyceride. Subjects who lost at least 5% of their body weight had significantly greater reduction in serum triglyceride and hepatic steatosis than those with <5% body weight loss. Enterococcus and Klebsiella were reduced at the end of the trial while Coprococcus and Collinsella were increased. There were also significant baseline microbiome differences between patients who had at least 5% weight loss as compared to those that did not. Lachnoclostridium was positively associated with hepatic steatosis and Actinomyces was positively associated with hepatic steatosis and weight. Baseline microbiome profiles were able to predict which patients lost at least 5% of their body weight with an AUROC of 0.80. Conclusion: Calorie restriction alters the intestinal microbiome and improves hepatic steatosis in those who experience significant weight loss. Baseline microbiome differences predict weight loss on a calorie–restricted diet and are associated with improvement in hepatic steatosis, suggesting a role of the gut microbiome in mediating the clinical response to calorie restriction.
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影响因子：
17.1
作者：
Turnbaugh PJ;Ridaura VK;Faith JJ;Rey FE;Knight R;Gordon JI
通讯作者：
Gordon JI
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
3.7
作者：
Jun BG;Park WY;Park EJ;Jang JY;Jeong SW;Lee SH;Kim SG;Cha SW;Kim YS;Cho YD;Kim HS;Kim BS;Jin SY;Park S
通讯作者：
Park S
DOI：
10.1073/pnas.1219451110
发表时间：
2013-05-28
影响因子：
11.1
作者：
Everard, Amandine;Belzer, Clara;Cani, Patrice D.
通讯作者：
Cani, Patrice D.
影响因子：
29.4
作者：
Goldberg D;Ditah IC;Saeian K;Lalehzari M;Aronsohn A;Gorospe EC;Charlton M
通讯作者：
Charlton M