The archetypal gene transfer agent RcGTA is regulated via direct interaction with the enigmatic RNA polymerase omega subunit.
The archetypal gene transfer agent RcGTA is regulated via direct interaction with the enigmatic RNA polymerase omega subunit.
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DOI：
10.1016/j.celrep.2022.111183
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发表时间：
2022-08-09
期刊：
影响因子：
8.8
通讯作者：
Fogg, Paul C. M.
中科院分区：
文献类型：
--
作者：
Sherlock, David;Fogg, Paul C. M.
基因转移因子（GTAs）是一类类似病毒的微小颗粒，它们会不加选择地包装并转移其宿主细胞中存在的任何DNA，这对细菌的进化具有明显影响。最近发现了第一种直接控制GTA表达的转录调节因子GafA，但其作用机制一直不清楚。在此，我们证明GafA通过与RNA聚合酶ω亚基（Rpo - ω）直接相互作用来控制GTA基因表达，并且还通过一种不依赖Rpo - ω的机制对自身表达进行正调控。我们表明GafA是一种具有不同DNA和蛋白质结合结构域的模块化蛋白质。我们在红杆菌（Rhodobacter）的GafA中观察到的功能结构域也对应于生丝微菌目（Hyphomicrobiales）病原体中的双基因操纵子。这些数据使我们能够构建出一个关于GTA最完整的调控模型，并指出了α - 变形菌（Alphaproteobacteria）中RNA聚合酶募集和特异性转录激活的一种非典型机制。
荚膜红杆菌（Rhodobacter capsulatus）转录因子GafA是一种多结构域蛋白质
远端DNA结合结构域促进自身调控以及荚膜红杆菌GTA基因的表达
GafA通过与ω亚基相互作用控制RNA聚合酶启动子的选择
生丝微菌目中存在类似的gafA基因，但分裂为两个基因
舍洛克（Sherlock）和福格（Fogg）表明荚膜红杆菌转录因子GafA是一种多结构域蛋白质。其C末端与自身启动子结合以进行自身调控，中间区域通过ω亚基与RNA聚合酶直接相互作用，并且整个蛋白质是激活类病毒基因转移因子所必需的。
Gene transfer agents (GTAs) are small virus-like particles that indiscriminately package and transfer any DNA present in their host cell, with clear implications for bacterial evolution. The first transcriptional regulator that directly controls GTA expression, GafA, was recently discovered, but its mechanism of action has remained elusive. Here, we demonstrate that GafA controls GTA gene expression via direct interaction with the RNA polymerase omega subunit (Rpo-ω) and also positively autoregulates its own expression by an Rpo-ω-independent mechanism. We show that GafA is a modular protein with distinct DNA and protein binding domains. The functional domains we observe in Rhodobacter GafA also correspond to two-gene operons in Hyphomicrobiales pathogens. These data allow us to produce the most complete regulatory model for a GTA and point toward an atypical mechanism for RNA polymerase recruitment and specific transcriptional activation in the Alphaproteobacteria. Rhodobacter capsulatus transcriptional factor GafA is a multi-domain protein Distal DNA-binding domains facilitate autoregulation and expression of RcGTA genes GafA controls RNAP promoter selection via interaction with the omega subunit Comparable gafA genes are present in the Hyphomicrobiales but split into two genes Sherlock and Fogg show that the Rhodobacter capsulatus transcriptional factor GafA is a multi-domain protein. The C terminus binds to its own promoter for autoregulation, the central region directly interacts with RNA polymerase via the omega subunit, and the whole protein is required for activation of the virus-like gene transfer agent.
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影响因子：
3.6
作者：
Brimacombe, Cedric A.;Stevens, Aaron;Beatty, J. Thomas
通讯作者：
Beatty, J. Thomas
影响因子：
64.8
作者：
Jumper J;Evans R;Pritzel A;Green T;Figurnov M;Ronneberger O;Tunyasuvunakool K;Bates R;Žídek A;Potapenko A;Bridgland A;Meyer C;Kohl SAA;Ballard AJ;Cowie A;Romera-Paredes B;Nikolov S;Jain R;Adler J;Back T;Petersen S;Reiman D;Clancy E;Zielinski M;Steinegger M;Pacholska M;Berghammer T;Bodenstein S;Silver D;Vinyals O;Senior AW;Kavukcuoglu K;Kohli P;Hassabis D
通讯作者：
Hassabis D
DOI：
10.1006/bbrc.2002.6559
发表时间：
2002-03-08
影响因子：
3.1
作者：
Griffith, KL;Shah, IM;Wolf, RE
通讯作者：
Wolf, RE
影响因子：
3.2
作者：
Chain, Patrick S. G.;Lang, Dorothy M.;Tolmasky, Marcelo E.
通讯作者：
Tolmasky, Marcelo E.
影响因子：
4.4
作者：
Kaczmarczyk, Andreas;Vorholt, Julia A.;Francez-Charlot, Anne
通讯作者：
Francez-Charlot, Anne