The Meloidogyne javanica effector Mj2G02 interferes with jasmonic acid signalling to suppress cell death and promote parasitism in Arabidopsis.
The Meloidogyne javanica effector Mj2G02 interferes with jasmonic acid signalling to suppress cell death and promote parasitism in Arabidopsis.
复制标题
爪哇根结线虫效应子 Mj2G02 干扰茉莉酸信号传导，抑制拟南芥细胞死亡并促进寄生
DOI：
10.1111/mpp.13111
复制
发表时间：
2021-10
影响因子：
4.9
通讯作者：
Zhuo K
中科院分区：
文献类型：
--
作者：
Song H;Lin B;Huang Q;Sun T;Wang W;Liao J;Zhuo K
植物寄生线虫会对农作物造成毁灭性的破坏。这些线虫分泌抑制宿主免疫反应的效应物以增强其存活。在这项研究中，Mj 2G 02，从根结线虫的效应，描述。原位杂交和转录分析表明，Mj 2G 02在线虫感染的早期即有高表达，且仅在线虫腹下食管腺细胞中表达。在植物中，靶向Mj 2G 02的RNA干扰削弱了爪哇根结线虫的寄生，并且Mj 2G 02转基因拟南芥系显示出对爪哇根结线虫更敏感。利用农杆菌介导的瞬时表达系统和植物免疫应答试验，我们证明了Mj 2G 02定位于植物细胞核中，并且可以抑制Gpa 2/RBP-1诱导的细胞死亡。此外，通过RNA-Seq和定量逆转录PCR分析，我们表明Mj 2G 02能够干扰宿主茉莉酸（JA）信号通路。多个茉莉酸ZIM结构域（JAZ）基因显著上调，而JAR 1基因和四个JA响应基因MYC 3，UPI，THI2.1和WRKY 75显著下调。此外，HPLC分析显示，与野生型植物相比，Mj 2G 02-转基因拟南芥系中的内源茉莉酸-异亮氨酸（JA-Ile）水平显著降低。我们的研究结果表明，爪哇根结线虫效应子Mj 2G 02抑制植物免疫反应，从而促进线虫寄生。这一过程可能是由JA-Ile减少和JAZ增强介导的，以抑制JA应答基因。来自爪哇根结线虫（Meloidogyne javanica）的效应子Mj 2G 02减少JA‐Ile并促进JAZ蛋白抑制JA‐响应基因并损害植物免疫。
Plant‐parasitic nematodes can cause devastating damage to crops. These nematodes secrete effectors that suppress the host immune responses to enhance their survival. In this study, Mj2G02, an effector from Meloidogyne javanica, is described. In situ hybridization and transcriptional analysis showed that Mj2G02 was highly expressed in the early infection stages and exclusively expressed in the nematode subventral oesophageal gland cells. In planta RNA interference targeting Mj2G02 impaired M. javanica parasitism, and Mj2G02‐transgenic Arabidopsis lines displayed more susceptibility to M. javanica. Using an Agrobacterium‐mediated transient expression system and plant immune response assays, we demonstrated that Mj2G02 localized in the plant cell nuclei and could suppress Gpa2/RBP‐1‐induced cell death. Moreover, by RNA‐Seq and quantitative reverse transcription PCR analyses, we showed that Mj2G02 was capable of interfering with the host jasmonic acid (JA) signalling pathway. Multiple jasmonate ZIM‐domain (JAZ) genes were significantly upregulated, whereas the JAR1 gene and four JA‐responsive genes, MYC3, UPI, THI2.1, and WRKY75, were significantly downregulated. In addition, HPLC analysis showed that the endogenous jasmonoyl‐isoleucine (JA‐Ile) level in Mj2G02‐transgenic Arabidopsis lines was significantly decreased compared to that in wildtype plants. Our results indicate that the M. javanica effector Mj2G02 suppresses the plant immune response, therefore facilitating nematode parasitism. This process is probably mediated by a JA‐Ile reduction and JAZ enhancement to repress JA‐responsive genes. The effector Mj2G02 from Meloidogyne javanica reduces JA‐Ile and promotes JAZ proteins to repress JA‐responsive genes and impair the plant immunity.
登录
查看更多内容
影响因子：
9.4
作者：
Jaouannet, Maelle;Perfus-Barbeoch, Laetitia;Rosso, Marie-Noelle
通讯作者：
Rosso, Marie-Noelle
影响因子：
4.9
作者：
Huang, GZ;Dong, RH;Hussey, RS
通讯作者：
Hussey, RS
影响因子：
11.6
作者：
Clarke, JD;Volko, SM;Dong, XN
通讯作者：
Dong, XN
影响因子：
6.7
作者：
Danchin EG;Arguel MJ;Campan-Fournier A;Perfus-Barbeoch L;Magliano M;Rosso MN;Da Rocha M;Da Silva C;Nottet N;Labadie K;Guy J;Artiguenave F;Abad P
通讯作者：
Abad P
影响因子：
3.5
作者：
Ding, X;Shields, J;Hussey, RS
通讯作者：
Hussey, RS