Environmental salinity-induced shifts in sperm motility activation in Fundulus grandis
Environmental salinity-induced shifts in sperm motility activation in Fundulus grandis
复制标题
环境盐度引起的巨眼精子活力激活的变化
DOI：
10.1016/j.aquaculture.2011.10.023
复制
发表时间：
2012
期刊：
影响因子：
4.5
通讯作者：
Huiping Yang
中科院分区：
文献类型：
--
作者：
T. Tiersch;Huiping Yang
鱼类精子的运动激活通常响应于特定离子的水平或周围水和身体组织之间的渗透压差。一般来说，海水鱼类的精子通过增加渗透压（高渗盐度）而被激活，而淡水物种的精子则通过降低渗透压（低渗盐度）而被激活。这些狭盐性物种存在于相对稳定的环境中，然而，河口鱼类暴露于快速变化和广泛的盐度范围，通常导致包括身体（等渗）的外部渗透压。为研究大鲵精子对盐度变化的适应能力，将成年雄虫在0、5、10、20、35和50 ppt的盐度下驯化30 d。从鱼上解剖睾丸，用去离子水、各种渗透压（100- 1000 mOsmol/kg）的Hanks平衡盐溶液（HBSS）、无钙HBSS（无Ca 2 + HBSS）和氯化钠溶液（NaCl）活化精子。去离子水没有激活精子活力，无论驯化的盐度。与HBSS相比，无钙HBSS和NaCl在相同渗透压下激活精子活力的百分比显著降低。引起最高运动激活的渗透压显著不同（P<0.01），从300 mOsmol/kg（范围从200到500），对于从5 ppt，500 mOsmol/kg收集的精子（范围从200到800），对于从10 ppt，600 mOsmol/kg收集的精子20 ppt以下的精子密度为400 ~ 700 mOsmol/kg，35 ppt以下的精子密度为200 ~ 900 mOsmol/kg，50 ppt以下的精子密度为900 mOsmol/kg，600 ~ 1000 mOsmol/kg。运动性在暴露于HBSS 30 s后达到峰值，并在10 min内下降。当暴露于无Ca 2 + HBSS时，活动性表现出类似的初始模式，然而，精子在较低渗透压下在10分钟内获得活动性，表现出多个峰。这些结果表明，环境盐度能显著地影响雄鱼精子的行为。grandis在驯化30 d后就发生了显著变化。因此，这应被视为该物种精子研究中一个未被认识到的主要变量，并可被考虑用于优化体外受精和冷冻保存方案。这种现象是底鱼所特有的，还是广盐性鱼类的一个特征，目前还没有定论。
Motility activation of fish sperm typically responds to levels of specific ions or osmotic pressure differences between the surrounding water and body tissues. In general, the sperm of marine fishes are activated by an increase in osmotic pressure (hypertonic salinity), and that of freshwater species by a decrease (hypotonic salinity). These stenohaline species exist in relatively stable environments, however, estuarine fishes are exposed to rapidly changing and broad salinity ranges, often resulting in external osmotic pressures that include those of the body (isotonic). To assess the ability of Fundulus grandis sperm to adapt to changes in salinity, adult males were acclimated to salinities of 0, 5, 10, 20, 35, or 50ppt and held for 30d. The testes were dissected from the fish and sperm were activated with deionized water, various osmolalities (100–1000mOsmol/kg) of Hanks' balanced salt solution (HBSS), calcium-free HBSS (Ca2+-Free HBSS), and sodium chloride solution (NaCl). The deionized water did not activate sperm motility regardless of the acclimated salinity. Compared to HBSS, Ca2+-Free HBSS and NaCl activated sperm motility with a significantly lower percentage at the same osmolalities. The osmolality eliciting the highest motility activation was significantly different (P<0.01) among acclimated groups and shifted from 300mOsmol/kg (ranging from 200 to 500) for sperm collected from 5ppt, 500mOsmol/kg (ranging from 200 to 800) for sperm collected from 10ppt, 600mOsmol/kg (ranging from 400 to 700) for sperm collected from 20ppt, 800mOsmol/kg (ranging from 200 to 900) for sperm collected from 35ppt, and 900mOsmol/kg (ranging from 600 to 1000) for sperm collected from 50ppt. Motility peaked after 30s exposure to HBSS, and decreased over 10min. Motility exhibited a similar initial pattern when exposed to Ca2+-Free HBSS, however, the sperm gained motility at lower osmolalities over 10min, exhibiting multiple peaks. These results indicate that environmental salinity can significantly influence sperm behavior in adult males of F. grandis with substantial changes after only 30d of acclimation. As such, this should be considered as a major unrecognized variable in sperm research in this species and can be considered for use in optimizing protocols addressing in-vitro fertilization and cryopreservation. Whether this phenomenon is unique to Fundulus or is a characteristic of euryhaline fishes remains unresolved.
影响因子：
2.8
作者：
Yang H;Tiersch TR
通讯作者：
Tiersch TR
影响因子：
2.8
作者：
Yang, Huiping;Carmichael, Carrie;Tiersch, Terrence R.
通讯作者：
Tiersch, Terrence R.