Sperm motility initiation and duration in a euryhaline fish, medaka (Oryzias latipes).
Sperm motility initiation and duration in a euryhaline fish, medaka (Oryzias latipes).
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DOI：
10.1016/j.theriogenology.2009.03.007
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发表时间：
2009-08
期刊：
影响因子：
2.8
通讯作者：
Tiersch TR
中科院分区：
文献类型：
--
作者：
Yang H;Tiersch TR
青鳉是一种公认的生物医学研究的鱼类模型。了解配子的特性对于人工受精和精子冷冻保存等实验操作是必要的。本研究的目的是调查青鳉精子运动的启动，持续时间和保留的特点。首先，通过25至686 mOsm/kg的渗透压值启动运动性，其中包括去离子水和低渗、等渗和高渗Hanks平衡盐溶液。当渗透压<315 mOsm/kg时，活动精子的百分比>80%，并随着渗透压的增加而降低。这与大多数具有外部受精的鱼类不同，其中精子运动可以通过低渗（对于淡水鱼）或高渗（对于海鱼）溶液或通过改变特定离子如钾的浓度来启动（例如，在鲑鱼科中）。第二，在激活后，精子在4 °C储存期间保持持续运动，具有储备能力，长达1周。这也不同于其他外部运动的鱼，其中运动通常维持数秒至数分钟。第三，通过加入去离子水将渗透压摩尔浓度改变为46至68 mOsm/kg后，在4 ℃下储存24、48和72 h后，保持在274至500 mOsm/kg的精子的活力高于原始活力（P ≤ 0.035）。第四，添加葡萄糖对冷藏期间维持精子活力没有影响。据我们所知，这种结合的精子活力的特点是第一次在鱼类，可能是独特的青鳉或可能代表一种未描述的方式的精子行为在广盐性鱼类。
The medaka, Oryzias latipes, is a well-recognized fish model for biomedical research. An understanding of gamete characteristics is necessary for experimental manipulations such as artificial fertilization and sperm cryopreservation. The goal of this study was to investigate sperm characteristics of motility initiation, duration, and retention in medaka. First, motility was initiated by osmolality values ranging from 25 to 686 mOsm/kg, which included deionized water and hypotonic, isotonic, and hypertonic Hanks’ balanced salt solution. The percentage of motile sperm was >80% when osmolality was <315 mOsm/kg and decreased as osmolality increased. This is different from most fish with external fertilization in which sperm motility can be initiated by hypotonic (for freshwater fish) or hypertonic (for marine fish) solutions or by altering the concentration of specific ions such as potassium (e.g., in salmonids). Second, upon activation, the sperm remained continuously motile, with reserve capacity, for as long as 1 wk during storage at 4 °C. This was also different from other externally fertilizing fish, in which motility is typically maintained for seconds to several minutes. Third, after changing the osmolality to 46 to 68 mOsm/kg by adding deionized water, the motility of sperm held at 274 to 500 mOsm/kg was higher than the original motility (P ≤ 0.035) after 24, 48, and 72 h of storage at 4 °C. Fourth, the addition of glucose had no effect on maintaining sperm motility during refrigerated storage. To our knowledge, this combination of sperm motility characteristics is reported for the first time in fish and may be unique to medaka or may represent an undescribed modality of sperm behavior within euryhaline fish.
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