Novel DNA methylation targets in oral rinse samples predict survival of patients with oral squamous cell carcinoma.
Novel DNA methylation targets in oral rinse samples predict survival of patients with oral squamous cell carcinoma.
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DOI：
10.1016/j.oraloncology.2014.08.015
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发表时间：
2014-11
期刊：
影响因子：
4.8
通讯作者：
Kelsey, Karl T.
中科院分区：
文献类型：
--
作者：
Langevin, Scott M.;Butler, Rondi A.;Eliot, Melissa;Pawlita, Michael;Maccani, Jennifer Z. J.;McClean, Michael D.;Kelsey, Karl T.
本研究的目的是在口腔鳞状细胞癌（OSCC）患者的口腔冲洗液样本中鉴定新的与生存相关的生物标志物。我们使用来自88个OSCC肿瘤的公开甲基化阵列数据筛选推定的生存相关标记物。然后将Cox模型与来自大波士顿地区82例OSCC患者口腔冲洗样本中这些假定位点的甲基化阵列数据进行拟合。对在口腔冲洗液样本中复制的每个基因座设计焦磷酸测序测定，并应用于另外61名OSCC患者的口腔冲洗液样本验证集。我们在OSCC患者的口腔冲洗液样本中发现了7个与生存相关的甲基化标记，并通过焦磷酸测序验证了一个位于GABBR1体内的甲基化标记。通过这项研究鉴定的7个CpG基因座代表了OSCC患者新的预后生物标志物，可以使用无创口腔冲洗收集技术进行检测。
The objective of this study was to identify novel survival-associated biomarkers in oral rinse samples collected from patients with oral squamous cell carcinoma (OSCC). We screened for putative survival-associated markers using publicly available methylation array data from 88 OSCC tumors. Cox models were then fit to methylation array data restricted to these putative loci in oral rinse samples of 82 OSCC patients from greater Boston. Pyrosequencing assays were designed for each locus that replicated in the oral rinse samples and applied to a validation set of oral rinse samples from another 61 OSCC patients. We identified 7 survival-associated methylation markers in oral rinse samples from OSCC patients, and have validated one, located in the body of GABBR1, by pyrosequencing. The 7 CpG loci identified through this study represent novel prognostic biomarkers for patients with OSCC that can be detected using a non-invasive oral rinse collection technique.
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影响因子：
3.7
作者：
Li Y;Fu L;Wong AM;Fan YH;Li MX;Bei JX;Jia WH;Zeng YX;Chan D;Cheung KM;Sham P;Chua D;Guan XY;Song YQ
通讯作者：
Song YQ
DOI：
10.1158/1078-0432.ccr-11-0324
发表时间：
2011-07-15
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Carvalho AL;Henrique R;Jeronimo C;Nayak CS;Reddy AN;Hoque MO;Chang S;Brait M;Jiang WW;Kim MM;Claybourne Q;Goldenberg D;Khan Z;Khan T;Westra WH;Sidransky D;Koch W;Califano JA
通讯作者：
Califano JA
影响因子：
4.7
作者：
Langevin, Scott M.;Stone, Roslyn A.;Taioli, Emanuela
通讯作者：
Taioli, Emanuela
影响因子：
3.7
作者：
Cui Y;Ying Y;van Hasselt A;Ng KM;Yu J;Zhang Q;Jin J;Liu D;Rhim JS;Rha SY;Loyo M;Chan AT;Srivastava G;Tsao GS;Sellar GC;Sung JJ;Sidransky D;Tao Q
通讯作者：
Tao Q
影响因子：
8.4
作者：
López, M;Aguirre, JM;de Pancorbo, MM
通讯作者：
de Pancorbo, MM