The two-component system CpxAR is required for the high potassium stress survival of Actinobacillus pleuropneumoniae.
The two-component system CpxAR is required for the high potassium stress survival of Actinobacillus pleuropneumoniae.
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DOI：
10.3389/fmicb.2023.1259935
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发表时间：
2023
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
--
作者：
胸膜肺炎放线杆菌是引起猪传染性胸膜肺炎的重要呼吸道病原菌，给世界养猪业造成巨大的经济损失。高钾对细菌来说是一个不利的环境，不利于为细胞生长和分裂提供膨压。双组分系统CpxAR是细菌响应环境变化的重要调控系统，参与多种生物学活动，如抗生素抗性、周质蛋白折叠、肽聚糖代谢等，但目前对CpxAR在高钾胁迫下的作用知之甚少。胸膜肺炎在这里，我们发现CpxAR对A.高钾（K+）胁迫下的胸膜肺炎。qRT-PCR分析发现CpxAR正调控细胞分裂基因ftsEX。此外，我们还通过EMSA证明CpxR-P可以直接结合细胞分裂基因ftsE的启动子区。总之，我们的结果描述了CpxAR调节A的机制。通过上调细胞分裂蛋白FtsE和FtsX的表达，胸膜肺炎菌在高K+胁迫下存活。这些发现首次直接证明了CpxAR介导的高K+耐受性，并研究了详细的分子机制。
Actinobacillus pleuropneumoniae is an important respiratory pathogen, which can cause porcine contagious pleuropneumonia and lead to great economic losses to worldwide swine industry. High potassium is an adverse environment for bacteria, which is not conducive to providing turgor pressure for cell growth and division. Two-component system CpxAR is an important regulatory system of bacteria in response to environmental changes, which is involved in a variety of biological activities, such as antibiotic resistance, periplasmic protein folding, peptidoglycan metabolism and so on. However, little is known about the role of CpxAR in high potassium stress in A. pleuropneumoniae. Here, we showed that CpxAR is critical for cell division of A. pleuropneumoniae under high potassium (K+) stress. qRT-PCR analysis found that CpxAR positively regulated the cell division genes ftsEX. In addition, we also demonstrated that CpxR-P could directly bind the promoter region of the cell division gene ftsE by EMSA. In conclusion, our results described a mechanism where CpxAR adjusts A. pleuropneumoniae survival under high-K+ stress by upregulating the expression of the cell division proteins FtsE and FtsX. These findings are the first to directly demonstrate CpxAR-mediated high-K+ tolerance, and to investigate the detailed molecular mechanism.
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