PhcA and PhcR Regulate Ralsolamycin Biosynthesis Oppositely in Ralstonia solanacearum.
PhcA and PhcR Regulate Ralsolamycin Biosynthesis Oppositely in Ralstonia solanacearum.
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PhcA 和 PhcR 对青枯雷尔斯顿菌中拉霉素生物合成的调节相反
DOI：
10.3389/fpls.2022.903310
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
Ralsolamycin是青枯雷尔氏菌的次生代谢产物之一，参与了R.青枯菌属和真菌。Ralsolamycin的形成由RmyA（非核糖体肽合成酶）和RmyB（聚酮化合物合成酶）的双杂交合成酶催化。甲基转移酶PhcB催化3-OH MAME或3-OH PAME的形成，3-OH MAME或3-OH PAME是R.而PhcB正调控雷索霉素的生物合成。PhcS和PhcR的双组分系统可以响应这些QS信号并激活phcA表达。在此，我们通过实验证明，phcA的缺失（ΔphcA）实质上损害了R.结果表明，EP 1菌株能诱导植物病原真菌尖孢镰刀菌（Fusarium oxysporum f.）古巴糖（stran FOC 4）。然而，phcR的缺失显着增加了拉索尔霉素的生产和rmyA和rmyB的表达，phcR突变体表现出增强的能力，诱导厚垣孢子形成的FOC 4。电泳迁移率变动分析的结果表明，PhcA和PhcR结合rmy操纵子的启动子。综上所述，这些结果表明PhcA和PhcR都与rmy操纵子的启动子结合，但以相反的方式调节雷索霉素的生物合成。这一结果有助于我们进一步了解R.青枯菌属
Ralsolamycin, one of secondary metabolites in Ralstonia solanacearum, is known to be involved in crosstalk between R. solanacearum and fungi. Ralsolamycin formation is catalyzed by two-hybrid synthetases of RmyA (non-ribosomal peptide synthetase) and RmyB (polyketide synthase). A methyltransferase PhcB catalyzes formation of 3-OH MAME or 3-OH PAME, signals for the quorum sensing (QS) in R. solanacearum, while PhcB positively modulates ralsolamycin biosynthesis. A two-component system of PhcS and PhcR can response these QS signals and activate phcA expression. Here, we experimentally demonstrated that deletion of phcA (ΔphcA) substantially impaired the ralsolamycin production and expression of rmyA and rmyB in R. solanacearum strain EP1, and failed to induce chlamydospore formation of plant fungal pathogen Fusarium oxysporum f. cubense (stran FOC4). However, deletion of phcR significantly increased ralsolamycin production and expression of rmyA and rmyB, and phcR mutants exhibited enhanced ability to induce chlamydospore formation of FOC4. Results of the electrophoretic mobility shift assay suggested that both PhcA and PhcR bind to promoter of rmy operon. Taken together, these results demonstrated that both PhcA and PhcR bind to promoter of rmy operon, but regulate ralsolamycin biosynthesis in an opposite way. It could extend our knowledge on the sophisticated regulatory networks of ralsolamycin biosynthesis in R. solanacearum.
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系统型I菌株EP1的基因组分析揭示了与Ralstonia solanacearum物种复合物中其他菌株的显着差异。
影响因子：
5.2
作者：
Li P;Wang D;Yan J;Zhou J;Deng Y;Jiang Z;Cao B;He Z;Zhang L
通讯作者：
Zhang L
影响因子：
64.8
作者：
Salanoubat, M;Genin, S;Boucher, CA
通讯作者：
Boucher, CA
DOI：
10.1073/pnas.77.12.7347
发表时间：
1980-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
DITTA, G;STANFIELD, S;HELINSKI, DR
通讯作者：
HELINSKI, DR
影响因子：
3.2
作者：
Bhatt, G;Denny, TP
通讯作者：
Denny, TP
影响因子：
5.2
作者：
Baldeweg, Florian;Kage, Hirokazu;Nett, Markus
通讯作者：
Nett, Markus