High throughput sequencing approaches to mutation discovery in the mouse.
High throughput sequencing approaches to mutation discovery in the mouse.
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DOI：
10.1007/s00335-012-9424-0
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发表时间：
2012-10
期刊：
影响因子：
2.5
通讯作者：
Reinholdt, Laura G.
中科院分区：
文献类型：
--
作者：
Simon, Michelle M.;Mallon, Ann-Marie;Howell, Gareth R.;Reinholdt, Laura G.
Phenotype driven approaches in mice are powerful strategies for the discovery of genes, gene functions and for unravelling complex biological mechanisms. Traditional methods for mutation discovery are reliable and robust, but they can also be laborious and time consuming. Recently, high throughput sequencing (HTS) technologies have revolutionised the process of forward genetics in mice by paving the way to rapid mutation discovery. However, successful application of HTS for mutation discovery is relies heavily on the sequencing approach employed and strategies for data analysis. Here, we review current HTS applications and resources for mutation discovery and provide an overview of the practical considerations for HTS implementation and data analysis.
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