The adult zebrafish retina: In vivo optical sectioning with Confocal Scanning Laser Ophthalmoscopy and Spectral-Domain Optical Coherence Tomography.
The adult zebrafish retina: In vivo optical sectioning with Confocal Scanning Laser Ophthalmoscopy and Spectral-Domain Optical Coherence Tomography.
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DOI：
10.1016/j.exer.2016.10.001
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发表时间：
2016-12
影响因子：
3.4
通讯作者：
Perkins, Brian D.
中科院分区：
文献类型：
--
作者：
Bell, Brent A.;Yuan, Alex;Dicicco, Rose M.;Fogerty, Joseph;Lessieur, Emma M.;Perkins, Brian D.
非侵入性成像在眼科是一种无价的诊断工具。扫描激光检眼镜(SLO)和光谱域光学相干层析成像(SDOCT)这两种成像设备从临床领域出现，为研究人员提供活体动物眼睛形态的实时图像。我们以互补的方式利用这两种独立的成像方式对成年斑马鱼的视网膜进行体内光学切片。由于斑马鱼透镜的光学功率非常高，共焦景深很窄，可以看到特定视网膜层的详细正面视图，包括圆锥体马赛克。此外，我们证明了SLO观测到的自然反射率和荧光特征都可以与SDOCT获得的轴向深度信息相结合。这些成像方法可以用来筛选眼部表型，并以非侵入性方式监测视网膜病理。
Non-invasive imaging is an invaluable diagnostic tool in ophthalmology. Two imaging devices, the scanning laser ophthalmoscope (SLO) and spectral domain optical coherence tomography (SDOCT), emerged from the clinical realm to provide research scientists with a real-time view of ocular morphology in living animals. We utilized these two independent imaging modalities in a complementary manner to perform in vivo optical sectioning of the adult zebrafish retina. Due to the very high optical power of the zebrafish lens, the confocal depth of field is narrow, allowing for detailed en face views of specific retinal layers, including the cone mosaic. Moreover, we demonstrate that both native reflectance, as well as fluorescent features observed by SLO, can be combined with axial in-depth information obtained by SDOCT. These imaging approaches can be used to screen for ocular phenotypes and monitor retinal pathology in a non-invasive manner.
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影响因子：
--
作者：
Alvarez Y;Cederlund ML;Cottell DC;Bill BR;Ekker SC;Torres-Vazquez J;Weinstein BM;Hyde DR;Vihtelic TS;Kennedy BN
通讯作者：
Kennedy BN
影响因子：
1.8
作者：
CHAUDHURI, A;HALLETT, PE;PARKER, JA
通讯作者：
PARKER, JA
影响因子：
4.4
作者：
DiCicco, Rose M.;Bell, Brent A.;Yuan, Alex
通讯作者：
Yuan, Alex
影响因子：
3.4
作者：
Bell, Brent A.;Kaul, Charles;Hollyfield, Joe G.
通讯作者：
Hollyfield, Joe G.
影响因子：
3.7
作者：
Collery RF;Veth KN;Dubis AM;Carroll J;Link BA
通讯作者：
Link BA