Measurements of the binding force between the Helicobacter pylori adhesin BabA and the Lewis b blood group antigen using optical tweezers.
Measurements of the binding force between the Helicobacter pylori adhesin BabA and the Lewis b blood group antigen using optical tweezers.
复制标题
使用光镊测量幽门螺杆菌粘附素 BabA 和 Lewis b 血型抗原之间的结合力。
DOI：
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发表时间：
2005
影响因子：
3.5
通讯作者：
S. Schedin
中科院分区：
文献类型：
--
作者：
O. Björnham;Erik Fällman;O. Axner;Jörgen Ohlsson;U. Nilsson;T. Borén;S. Schedin
幽门螺杆菌是一种在世界范围内传播的细菌，可引起持续性感染和慢性炎症，并可发展为胃炎和消化性溃疡疾病。它在其表面上表达几种粘附素蛋白，其与胃上皮中的特异性受体结合。最著名的粘附素是BabA，先前已显示其特异性结合岩藻糖基化血型抗原刘易斯B（LeB）。BabA和Leb抗原之间的粘附力进行了研究，在这项工作中，并通过光镊进行评估。开发了一种用于原位测量单个细菌与涂覆有Leb的珠之间的相互作用力的模型系统。据发现，在该模型系统中的脱粘力，测量的加载速率约为100 pN，范围从20到200 pN。脱粘力主要表现为基本力的倍数，其被确定为25+/-1.5 pN，并被鉴定为单个BabA-Leb结合的解结合力。它的结论是，粘附一般是由少量的结合（最常见的1至4）介导的，尽管细菌和珠之间的接触表面包括显着更多的结合位点。
Helicobacter pylori is a world-wide spread bacterium that causes persistent infections and chronic inflammations that can develop into gastritis and peptic ulcer disease. It expresses several adhesin proteins on its surface that bind to specific receptors in the gastric epithelium. The most well-known adhesin is BabA, which has previously been shown to bind specifically to the fucosylated blood group antigen Lewis b (Leb). The adhesion forces between BabA and the Leb antigen are investigated in this work and assessed by means of optical tweezers. A model system for in situ measurements of the interaction forces between individual bacteria and beads coated with Leb is developed. It is found that the de-adhesion force in this model system, measured with a loading rate of approximately 100 pNs, ranges from 20 to 200 pN. The de-adhesion force appears predominantly as multiples of an elementary force, which is determined to 25+/-1.5 pN and identified as the unbinding force of an individual BabA-Leb binding. It is concluded that adhesion in general is mediated by a small number of bindings (most often 1 to 4) despite that the contact surface between the bacterium and the bead encompassed significantly more binding sites.
影响因子：
3.4
作者：
Evans, E;Ritchie, K
通讯作者：
Ritchie, K
影响因子：
2.9
作者：
STAROS, JV;WRIGHT, RW;SWINGLE, DM
通讯作者：
SWINGLE, DM
影响因子：
56.9
作者：
BOREN, T;FALK, P;NORMARK, S
通讯作者：
NORMARK, S