Exfoliated oral cell messenger RNA: suitability for biomarker studies.
Exfoliated oral cell messenger RNA: suitability for biomarker studies.
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脱落的口腔细胞信使 RNA：适用于生物标志物研究。
DOI：
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
B. Braakhuis
中科院分区：
文献类型：
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作者：
I. Klaassen;M. Copper;R. Brakenhoff;S. Smeets;G. Snow;B. Braakhuis
化学预防试验的有效性可以通过使用作为替代终点的致癌生物标志物来提高。本研究的目的是评估从口腔脱落细胞中分离的mRNA用于上呼吸消化道癌化学预防的生物标志物研究的前景。当使用反转录-PCR与Southern印迹和杂交相结合时，可以检测到仅来自五个细胞的转录物。用定量RNase保护试验，我们只能检测到高丰度的转录本。通过北方印迹法验证RNA的完整性，其显示不同程度的降解，取决于所研究的基因。有趣的是，虽然发现特定的转录物在一定程度上是完整的，但rRNA似乎被完全降解，这表明在终末分化的口腔上皮细胞中存在特定的蛋白质合成关闭机制。总而言之，这种差异RNA降解使得在脱落的口腔细胞中测定的大多数基因的转录水平的准确测量不可靠。由于这种RNA降解过程可能在细胞从组织脱落之前就开始了，因此应谨慎解释口腔组织活检中转录水平的测量结果。
The efficacy of chemoprevention trials can be improved by the use of biomarkers of carcinogenesis that serve as surrogate end points. The aim of this study was to assess the perspectives of using mRNA isolated from oral exfoliated cells for biomarker research in chemoprevention of upper aerodigestive tract cancer. When using reverse transcription-PCR in combination with Southern blotting and hybridization, it was possible to detect transcripts from only five cells. With the quantitative RNase protection assay, we could only detect highly abundant transcripts. The integrity of the RNA was verified by Northern blotting, which showed a variable degree of degradation, depending on the gene studied. Interestingly, although specific transcripts were found to be intact to a certain extent, the rRNA appeared to be completely degraded, suggesting that a specific protein synthesis shut-off mechanism exists in terminally differentiated oral epithelial cells. Altogether, this differential RNA degradation makes accurate measurement of transcript levels of most genes, as determined in exfoliated oral cells, unreliable. Because this RNA degradation process is likely to start before the cells are shed from the tissue, the results of measurements of transcript levels in biopsies of oral tissue should be interpreted with caution.
使用粪便信使 RNA 无创检测结肠癌的假定生物标志物。
DOI：
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发表时间：
1995
期刊：
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology
影响因子：
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作者：
Davidson,LA;Jiang,YH;Lupton,JR;Chapkin,RS
通讯作者：
Chapkin,RS
影响因子：
11.2
作者：
X. C. Xu;Jae Y. Ro;J. S. Lee;Dong M. Shin;W. Hong;R. Lotan
通讯作者：
X. C. Xu;Jae Y. Ro;J. S. Lee;Dong M. Shin;W. Hong;R. Lotan
与吸烟和上呼吸消化道癌相关的致癌生物标志物。
DOI：
10.1002/jcb.240531005
发表时间：
1993
期刊：
Journal of cellular biochemistry. Supplement
影响因子：
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作者：
Hecht,SS;Carmella,SG;Murphy,SE;Foiles,PG;Chung,FL
通讯作者：
Chung,FL