Structural basis of template strand deoxyuridine promoter recognition by a viral RNA polymerase.
Structural basis of template strand deoxyuridine promoter recognition by a viral RNA polymerase.
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DOI：
10.1038/s41467-022-31214-6
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发表时间：
2022-06-20
影响因子：
16.6
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中科院分区：
文献类型：
--
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细菌RNA聚合酶（rnap）启动子的识别由sigma亚基控制。sigma识别的关键序列基序，−10启动子元件，位于转录起始位点上游约10个核苷酸的双链DNA分子的非模板链上。在这里，我们解释了噬菌体AR9非病毒粒子RNAP (nvRNAP)，细菌RNAP的同源物，识别模板链中含有脱氧尿苷启动子的- 10元素的机制。AR9 sigma样亚基、nvRNAP酶核和模板链共同形成两个核苷酸碱基接受袋，其形状决定了对保守脱氧尿苷的需求。AR9 sigma样亚基中的单个氨基酸替换允许这些口袋中的一个接受胸腺嘧啶，从而扩大启动子共识。我们的工作表明，病毒可以进化宿主衍生的多亚基酶，使其自身基因的转录独立于宿主。启动子识别是DNA转录为RNA起始的关键步骤。在这里，作者描述了一种新的机制，通过噬菌体编码的RNA聚合酶识别含有脱氧尿苷而不是胸腺嘧啶的病毒启动子。
Recognition of promoters in bacterial RNA polymerases (RNAPs) is controlled by sigma subunits. The key sequence motif recognized by the sigma, the −10 promoter element, is located in the non-template strand of the double-stranded DNA molecule ~10 nucleotides upstream of the transcription start site. Here, we explain the mechanism by which the phage AR9 non-virion RNAP (nvRNAP), a bacterial RNAP homolog, recognizes the −10 element of its deoxyuridine-containing promoter in the template strand. The AR9 sigma-like subunit, the nvRNAP enzyme core, and the template strand together form two nucleotide base-accepting pockets whose shapes dictate the requirement for the conserved deoxyuridines. A single amino acid substitution in the AR9 sigma-like subunit allows one of these pockets to accept a thymine thus expanding the promoter consensus. Our work demonstrates the extent to which viruses can evolve host-derived multisubunit enzymes to make transcription of their own genes independent of the host. Promoter recognition is a critical step in the initiation of transcription of DNA to RNA. Here, the authors describe a novel mechanism by which a phage-encoded RNA polymerase recognizes viral promoters containing deoxyuridines instead of thymidines.
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影响因子：
4.1
作者：
BENNETT, CH
通讯作者：
BENNETT, CH
影响因子：
2.8
作者：
BEUTLER, TC;MARK, AE;VANGUNSTEREN, WF
通讯作者：
VANGUNSTEREN, WF
影响因子：
64.5
作者：
Feklistov A;Darst SA
通讯作者：
Darst SA
影响因子：
4.4
作者：
Harrach, Michael F.;Drossel, Barbara
通讯作者：
Drossel, Barbara
影响因子：
5.4
作者：
Ceyssens, Pieter-Jan;Minakhin, Leonid;Lavigne, Rob
通讯作者：
Lavigne, Rob