Energetic evolution of cellular Transportomes.
Energetic evolution of cellular Transportomes.
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DOI：
10.1186/s12864-018-4816-5
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发表时间：
2018-05-30
期刊：
影响因子：
4.4
通讯作者：
Borodina I
中科院分区：
文献类型：
--
作者：
Darbani B;Kell DB;Borodina I
转运蛋白介导物质跨活细胞膜的转运。许多运输过程在能量上是昂贵的，并且电池使用其能量的20%至60%来为运输器提供动力。我们假设，可能有一个进化选择压力较低的能量转运。我们对细菌、古细菌和真核生物界转运体的组成重塑进行了全基因组分析。我们发现，ABC转运蛋白的份额在细菌和古细菌中要高得多（约。比原始真核生物（13%），藻类和植物（10%）和真菌和动物（5-6%）。这种减少通过二级转运蛋白和离子通道的增加来补偿。离子通道的份额在动物中特别高（约。30%的转运体）和藻类和植物（约。13%），相比之下，细菌和古细菌只有6- 7%。因此，我们的研究结果表明，作为从原核生物向真核生物过渡的一部分，我们倾向于选择低能量需求的转运蛋白（离子通道和载体），而不是更高能量消耗的转运蛋白类（ATP依赖家族，特别是ABC）。转运体分析还表明，包括新立克次氏体和Neorickettsia sennetsu在内的七种细菌物种可能是真核生物中的线粒体的起源，这是基于现代线粒体溶质载体的明确同源物在其中的遗传学限制。结果表明，真核生物的转运体强烈进化向更高的能量效率，ATP依赖的转运减少和二级转运和离子通道增殖。这些变化可能在执行能量昂贵的细胞功能的组织的发育中很重要。本文的在线版本（10.1186/s12864-018-4816-5）包含补充材料，可供授权用户使用。
Transporter proteins mediate the translocation of substances across the membranes of living cells. Many transport processes are energetically expensive and the cells use 20 to 60% of their energy to power the transportomes. We hypothesized that there may be an evolutionary selection pressure for lower energy transporters. We performed a genome-wide analysis of the compositional reshaping of the transportomes across the kingdoms of bacteria, archaea, and eukarya. We found that the share of ABC transporters is much higher in bacteria and archaea (ca. 27% of the transportome) than in primitive eukaryotes (13%), algae and plants (10%) and in fungi and animals (5–6%). This decrease is compensated by an increased occurrence of secondary transporters and ion channels. The share of ion channels is particularly high in animals (ca. 30% of the transportome) and algae and plants with (ca. 13%), when compared to bacteria and archaea with only 6–7%. Therefore, our results show a move to a preference for the low-energy-demanding transporters (ion channels and carriers) over the more energy-costly transporter classes (ATP-dependent families, and ABCs in particular) as part of the transition from prokaryotes to eukaryotes. The transportome analysis also indicated seven bacterial species, including Neorickettsia risticii and Neorickettsia sennetsu, as likely origins of the mitochondrion in eukaryotes, based on the phylogenetically restricted presence therein of clear homologues of modern mitochondrial solute carriers. The results indicate that the transportomes of eukaryotes evolved strongly towards a higher energetic efficiency, as ATP-dependent transporters diminished and secondary transporters and ion channels proliferated. These changes have likely been important in the development of tissues performing energetically costly cellular functions. The online version of this article (10.1186/s12864-018-4816-5) contains supplementary material, which is available to authorized users.
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DOI：
10.1126/science.aad8266
发表时间：
2016-08-26
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Chen Y;Clarke OB;Kim J;Stowe S;Kim YK;Assur Z;Cavalier M;Godoy-Ruiz R;von Alpen DC;Manzini C;Blaner WS;Frank J;Quadro L;Weber DJ;Shapiro L;Hendrickson WA;Mancia F
通讯作者：
Mancia F
影响因子：
3.5
作者：
Cross, RL;Müller, V
通讯作者：
Müller, V
影响因子：
5.4
作者：
Almen, Markus Sallman;Nordstrom, Karl J. V.;Schioth, Helgi B.
通讯作者：
Schioth, Helgi B.
DOI：
10.1073/pnas.1206390109
发表时间：
2012-11-06
影响因子：
11.1
作者：
Fonseca-Azevedo, Karina;Herculano-Houzel, Suzana
通讯作者：
Herculano-Houzel, Suzana
影响因子：
4.1
作者：
Bröer, S;Bröer, A;Deitmer, JW
通讯作者：
Deitmer, JW