Overexpression of AtEDT1/HDG11 in Chinese Kale (Brassica oleracea var. alboglabra) Enhances Drought and Osmotic Stress Tolerance.
Overexpression of AtEDT1/HDG11 in Chinese Kale (Brassica oleracea var. alboglabra) Enhances Drought and Osmotic Stress Tolerance.
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AtEDT1/HDG11 在羽衣甘蓝 (Brassica oleracea var. alboglabra) 中的过表达增强了干旱和渗透胁迫耐受性
DOI：
10.3389/fpls.2016.01285
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发表时间：
2016
影响因子：
5.6
通讯作者：
Lei J
中科院分区：
文献类型：
--
作者：
Zhu Z;Sun B;Xu X;Chen H;Zou L;Chen G;Cao B;Chen C;Lei J
植物不断受到环境胁迫的挑战，包括干旱和高盐。如何在不降低产量的前提下提高作物对干旱和渗透胁迫的耐受性是作物改良的一个重大挑战。拟南芥增强的耐旱性1/同源域Glabrous 11（AtEDT 1/HDG 11）是IV类HD-Zip家族的蛋白，已被证明能显著提高拟南芥、水稻和辣椒的耐旱性。在这里，我们报告AtEDT 1/HDG 11赋予干旱和渗透胁迫耐受性的芥蓝。AtEDT 1/HDG 11-过表达株系表现出生长素过量生产的表型，例如长的下胚轴、高的茎、更多的根毛和更大的根系结构。与未转化的对照相比，转基因株系具有显著降低的气孔密度。干旱和渗透胁迫后，转基因芥蓝叶片中脯氨酸（Pro）含量和活性氧清除酶活性显著增加，与野生芥蓝相比，差异显著。更重要的是，AtEDT 1/HDG 11-过表达导致脱落酸（阿坝）过敏，导致阿坝抑制剂发芽和诱导气孔关闭。与观察到的表型一致，生长素，阿坝和应力相关基因的表达水平也改变了正常和/或应力条件下。进一步分析表明，AtEDT 1/HDG 11作为转录因子，可以靶向生长素合成基因YUCC 6和阿坝反应基因ABI 3和ABI 5。总的来说，我们的研究结果提供了一个新的见解AtEDT 1/HDG 11在提高非生物胁迫抗性通过生长素和ABA介导的信号反应在中国羽衣甘蓝中的作用。
Plants are constantly challenged by environmental stresses, including drought and high salinity. Improvement of drought and osmotic stress tolerance without yield decrease has been a great challenge in crop improvement. The Arabidopsis ENHANCED DROUGHT TOLERANCE1/HOMEODOMAIN GLABROUS11 (AtEDT1/HDG11), a protein of the class IV HD-Zip family, has been demonstrated to significantly improve drought tolerance in Arabidopsis, rice, and pepper. Here, we report that AtEDT1/HDG11 confers drought and osmotic stress tolerance in the Chinese kale. AtEDT1/HDG11-overexpression lines exhibit auxin-overproduction phenotypes, such as long hypocotyls, tall stems, more root hairs, and a larger root system architecture. Compared with the untransformed control, transgenic lines have significantly reduced stomatal density. In the leaves of transgenic Chinese kale plants, proline (Pro) content and reactive oxygen species-scavenging enzyme activity was significantly increased after drought and osmotic stress, particularly compared to wild kale. More importantly, AtEDT1/HDG11-overexpression leads to abscisic acid (ABA) hypersensitivity, resulting in ABA inhibitor germination and induced stomatal closure. Consistent with observed phenotypes, the expression levels of auxin, ABA, and stress-related genes were also altered under both normal and/or stress conditions. Further analysis showed that AtEDT1/HDG11, as a transcription factor, can target the auxin biosynthesis gene YUCC6 and ABA response genes ABI3 and ABI5. Collectively, our results provide a new insight into the role of AtEDT1/HDG11 in enhancing abiotic stress resistance through auxin- and ABA-mediated signaling response in Chinese kale.
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影响因子：
64.8
作者：
Blilou, I;Xu, J;Scheres, B
通讯作者：
Scheres, B
影响因子：
27.5
作者：
Kim, Jeong Im;Baek, Dongwon;Yun, Dae-Jin
通讯作者：
Yun, Dae-Jin
影响因子：
11.4
作者：
Cai, Xiao-Teng;Xu, Ping;Xiang, Cheng-Bin
通讯作者：
Xiang, Cheng-Bin
影响因子：
11.6
作者：
Khosla, Aashima;Paper, Janet M.;Schrick, Kathrin
通讯作者：
Schrick, Kathrin
影响因子：
7.4
作者：
Battaglia, Marina;Olvera-Carrillo, Yadira;Covarrubias, Alejandra A.
通讯作者：
Covarrubias, Alejandra A.