Portable two-color photometer based on paired light emitter detector diodes and its application to the determination of paraquat and diquat
Portable two-color photometer based on paired light emitter detector diodes and its application to the determination of paraquat and diquat
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基于成对光发射检测二极管的便携式双色光度计及其在百草枯和敌草快测定中的应用
DOI：
10.1016/j.microc.2021.106777
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发表时间：
2021
影响因子：
4.8
通讯作者：
Kaneta Takashi
中科院分区：
文献类型：
--
作者：
Seetasang Sasikarn;Kaneta Takashi
本文介绍了一种使用新开发的配备两种颜色的配对光发射器检测器（PEDD）的便携式光度计来测定百草枯和双菊枯的方法。本研究中采用的比色测量方法包括：1)二硫苏糖醇和双喹特之间的氧化还原反应，产生双喹特自由基的红色特征；2)二硫代硫酸钠与双喹特或百草枯之间的氧化还原反应，分别产生双喹特和百草枯自由基的绿色和蓝色。在固态中添加二硫代硝酸钠或二硫代苏糖醇可提供自由基的可重复吸收，防止试剂在溶液中分解，并简化试剂的处理。用一对最大发射波长为472 nm的蓝色led成功检测了双硫苏糖醇产生的双魁特自由基（λmax= 495 nm），用一对最大发射波长为609 nm的橙色led成功检测了双硫苏糖钠还原的百草枯（λmax= 603 nm）和双魁特（λmax= 771 nm）的自由基。该方法包括测量472 nm处的双quat自由基，估计609 nm处双quat自由基的吸光度，然后从609 nm处的总吸光度中减去估计的双quat自由基的吸光度来确定百草枯自由基。在472 nm处测定的双魁特自由基和609 nm处测定的双魁特和百草枯自由基的3条校准曲线上，该方法的线性回归（r2）均大于0.99。3条校准曲线的日间（n = 3）和日间（n = 3）精度均小于或等于5%。通过对市售除草剂中百草枯和双草枯自由基的分析，与高效液相色谱的标准方法进行比较，证明了该方法的可靠性。
Here we describe a methodology for the determination of paraquat and diquat using a newly developed portable photometer equipped with two colors of paired light emitter detector diodes (PEDD). The colorimetric measurements employed in this work include the redox reactions between 1) dithiothreitol and diquat to produce the red color characteristic of a diquat radical and 2) between sodium dithionite and either diquat or paraquat that results in the green and blue colors of diquat and paraquat radicals, respectively. The addition of sodium dithionite or dithiothreitol in a solid-state provides reproducible absorbance of the radicals, prevents decomposition of the reagents in a solution, and simplifies handling of the reagents. The diquat radical produced by dithiothreitol (λmax= 495 nm) was successfully detected by using a pair of blue LEDs with a maximum emission wavelength at 472 nm while the radicals of paraquat (λmax= 603 nm) and diquat (λmax= 771 nm) reduced by sodium dithionite were measured by a pair of orange LEDs with a maximum emission wavelength of 609 nm. The proposed method consists of measuring diquat radicals at 472 nm, estimating the absorbance of diquat radicals at 609 nm, and subtracting the estimated absorbance of diquat radicals from the total absorbance at 609 nm to determine paraquat radicals. The developed method yielded examples of excellent linear regression (r2) of more than 0.99 in three calibration curves of the radicals measured at 472 nm for diquat radicals and measured at 609 nm for both diquat and paraquat radicals. The intra-day (n = 3) and inter-day (n = 3) precision of three calibration curves were less than or equal to 5%. By comparison with the standard method of high-performance liquid chromatography, the reliability of the proposed method was proven via the analysis of paraquat and diquat radicals in a commercially available herbicide.
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