RLM1, Encoding an R2R3 MYB Transcription Factor, Regulates the Development of Secondary Cell Wall in Rice.
RLM1, Encoding an R2R3 MYB Transcription Factor, Regulates the Development of Secondary Cell Wall in Rice.
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RLM1编码一个R2R3MYB转录因子，调控水稻次生细胞壁的发育。
DOI：
10.3389/fpls.2022.905111
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
叶片形态是水稻理想株型的重要组成部分。到目前为止，已经克隆了许多影响水稻叶片形态的调控基因，并初步阐明了其潜在的分子调控机制。然而，叶片形态发生与株型的精细调控关系仍很难确定。本研究获得了一个卷叶突变体rlm1-D，该突变体受一对显性核基因控制。细胞学观察表明，rlm1主要是由次生细胞壁的异常沉积引起的。分子证据表明，MYB型转录因子LOC_Os05g46610的异位表达是导致rlm1-D表型的原因。一系列实验，包括以转录因子为中心的技术、DNA结合分析和凝胶迁移率改变分析，证实RLM1可以与水稻木质素生物合成关键基因OsCAD2的启动子结合。确定了RLM1的相互作用伙伴OsMAPK10。多种生化分析证实OsMAPK10与RLM1相互作用。OsMAPK10正向调控水稻叶片和茎中的木质素含量。此外，OsMAPK10还参与了RLM1下游靶基因的激活。特别是，RLM1在成熟植株阶段仅在茎中表达。RLM1基因敲除系在没有其他不利农艺性状损失的情况下增产11%以上，具有较大的实际应用价值。构建了控制SCW的MAPK-MYB-OsCAD2遗传调控网络，为形成理想株型、提高水稻产量提供了理论意义和实用价值。
Leaf morphology is an important component of rice ideal plant type. To date, many regulatory genes influencing leaf morphology in rice have been cloned, and their underlying molecular regulatory mechanism has been preliminarily clarified. However, the fine regulation relationship of leaf morphogenesis and plant type remains largely elusive. In this study, a rolling-leaf mutant, named rlm1-D, was obtained and controlled by a pair of dominant nuclear genes. Cytological observations revealed that the rlm1 was mainly caused by abnormal deposition of secondary cell walls. Molecular evidence showed ectopic expression of a MYB-type transcription factor LOC_Os05g46610 was responsible for the phenotype of rlm1-D. A series of experiments, including the transcription factor-centered technology, DNA-binding assay, and electrophoretic mobility shift assay, verified that RLM1 can bind to the promoter of OsCAD2, a key gene responsible for lignin biosynthesis in rice. An interacting partner of RLM1, OsMAPK10, was identified. Multiple biochemical assays confirmed that OsMAPK10 interacted with RLM1. OsMAPK10 positively regulated the lignin content in the leaves and stems of rice. Moreover, OsMAPK10 contributes to RLM1 activation of downstream target genes. In particular, RLM1 is exclusively expressed in the stems at the mature plant stage. The yield of RLM1 knockdown lines increased by over 11% without other adverse agricultural trait penalties, indicating great practical application value. A MAPK-MYB-OsCAD2 genetic regulatory network controlling SCW was proposed, providing a theoretical significance and practical value for shaping the ideal plant type and improving rice yield.
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影响因子：
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作者：
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DOI：
10.1073/pnas.1516110113
发表时间：
2016-10-18
影响因子：
11.1
作者：
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通讯作者：
Heisler, Marcus G.